Pengfei
Li
a,
Mingying
Peng
*a,
Lothar
Wondraczek
b,
Yanqi
Zhao
a and
Bruno
Viana
c
aThe China-Germany Research Center for Photonic Materials and Devices, The State Key Laboratory of Luminescent Materials and Devices, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China. E-mail: pengmingying@scut.edu.cn
bOtto Schott Institute of Materials Research, University of Jena, 07743 Jena, Germany
cIRCP Chimie ParisTech, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
First published on 17th February 2015
For specific techniques of bioimaging or photodynamic tumor therapy, it is highly desirable to find a luminescent material with emission wavelengths within the region of transparency of biological tissue, i.e., 650 – 1300 nm, and with multiple-hour luminescence afterglow. Here, we demonstrate red to near-infrared persistent luminescence from Mn2+ doped sodium gallium aluminum germanate glasses, covering the spectral region of 600 to 800 nm. Persistent luminescence from octahedral Mn2+ is associated with the presence of electronic defects with depths of 0.71 eV, 0.80 eV and 1.06 eV, which de-trap thermally at ambient temperature. The process of de-trapping occurs over a time of more than 40 h. When nanocrystalline high-albite Na(Al,Ga)Ge3O8 is precipitated from the supercooled melt through controlled thermal annealing, the red afterglow can be preserved, and the afterglow duration can be further extended to more than 100 h. This originates from the incorporation of Mn2+ on octahedral lattice sites with a more ionic bonding character in the compound, and a significant increase of defect density with, at the same time, decreasing depth, i.e., 0.70 eV, 0.75 eV and 0.85 eV. The observed properties suggest that the Mn2+ doped Na(Al,Ga)Ge3O8 nanocrystal may provide a new solution to in vivo bioimaging or photodynamic tumor therapy.
The two essential applications necessitate the persistent luminescence materials with suitable emission wavelengths and afterglow time. The emission should better occur in the transparency window of biological tissue, i.e., in the spectral range of ∼650–1300 nm. The afterglow time should better be longer than a few hours. It is the aim of our work to find such candidate materials.
This actually has stimulated the search for promising materials, and it has led to the series of findings. These include rare earth (RE) or transition metal (TM) doped persistent phosphors.12–23 For RE-based phosphors, latest advances are on Eu2+-doped nitrides and Eu3+-doped tungstates, e.g., M2Si5N8:Eu2+ or MWO4:Eu3+.22,23 Their emission, however, peaks at wavelengths below 650 nm, and the afterglow time is typically less than one hour.23 So far as we know, the most promising phosphors are trivalent chromium doped gallates.4,11,12,21 Pan et al.4 reported an afterglow duration longer than 360 h as Zn and Ge were substituted for Ga sites. In those materials, luminescence covers the spectral range of 680 to 750 nm, due to the Cr3+ transition 2E → 4A2 in the octahedral surroundings.
For TM-based persistent phosphors, there are also reports on Mn2+ doped persistent phosphors but mainly centering on silicate, germanate or phosphates.9,21,24–27 The luminescence mostly occurs over the spectral range of 600 to 750 nm, and it comes from the octahedral sites of Mn2+ (VIMn2+) once doped into the crystalline phase.28 Mn2+ doped glasses were also reported with red emissions with lifetime in the millisecond regime such as zinc borosilicates and germanates.29–34 As the glass samples were devitrified, it has been found that Mn2+ emission will turn into green emission32–35 This is because usually tetrahedral cation sites are predominant in the crystals precipitated inside glass, and Mn2+ ions will be driven into the tetrahedral lattice sites in the weak crystal field during the crystallization process. So far, the afterglow of VIMn2+-related red photoemission is seldom longer than one hour either in crystals, glasses or glass ceramics. There are also very few report on glass ceramics which can stabilize VIMn2+ emission centers in crystalline rather than the glass phase. Is it possible inside a glass to precipitate a crystalline phase which comprises octahedral host cationic sites in which Mn2+ ions prefer to reside? If so, will the Mn2+ luminescent afterglow be longer than one hour?
In this work, we select germanate glass as a target host because Mn2+ red afterglow has been found in the glass system and the glow duration has not been mentioned yet.33–37 We are looking for a glass and a glass ceramic system in which, upon devitrification, Mn2+ will remain in octahedral coordination, but will at least partially be incorporated into the crystal phase (rather than being accumulated in the residual glass phase). We select Mn2+ as the activator ions rather than Cr3+. This is partially because Mn2+ can show similar red to near infrared emission to Cr3+, and partially because Cr3+ containing materials once exposed to oxygen rich environments will tend to be oxidized perhaps into higher valence states such as Cr6+. This species is highly toxic and easily leads to cancer once entering into the human body. Since Cr3+ exhibits the outstanding persistent luminescence in gallate, we introduced gallium on purpose into the germanate glass and wish it could help improving Mn2+ afterglow properties.4,13,21,38 Besides, we added percentage of glass modifier ions Na+, which can breakdown the germanate glass network and therefore lower the melting temperature.
We show in the following that Mn2+-doped sodium gallium germanate glass indeed exhibit red persistence luminescence in the spectral range of 600 nm to 800 nm and afterglow can persist longer than 48 hours at a dopant content of 0.1% Mn2+. Out of our expectation is that the nanocrystalline Na(Ga,Al)Ge3O8 phase can be precipitated from the supercooled melt and it comprises of the sodium octahedral sites and the aluminum/germanium/gallium sites. Mn2+ doped glass ceramics can preserve the red emission of Mn2+ ions which substitute for sodium sites in Na(Ga,Al)Ge3O8 (see Fig. 1), and in the meanwhile, the emission intensity can be enhanced while the persistence of luminescence can be improved longer than 100 h. We have employed different techniques such as static and dynamic excitation and photoluminescence spectra, electron paramagnetic resonance spectrum, thermoluminescence, the time resolved emission spectrum, high resolution transmission electron microscopy, etc. to unravel how the red persistent luminescence of Mn2+ comes into being and why it can be significantly improved in glass ceramic samples, and evaluate whether it can find application in tumor bioimaging or photodynamic therapy.
The afterglow decay curves and thermoluminescence (TL) curves were measured with a FJ427A1 thermoluminescent dosimeter (CNNC Beijing Nuclear Instrument Factory). Before each measurement, the samples were first heated to 250 °C to completely de-trap all electrons and to heal-out eventual pre-existing (shallow) defects. An amount of 0.005 g of powder was weighed for each sample and exposed to 254 nm illumination from a mercury lamp at an output power of 60 W for 5 min. Subsequently, with a total delay time of 30 s after removal of the excitation source, photoluminescence decay curves were recorded. After a total delay of 10 min, TL curves where recorded. The range of the TL curves measurement was from room temperature (RT) to 250 °C with a scanning rate of 1 °C s−1. If not otherwise stated, all other measurements were performed at room temperature.
Fig. 2 Electron paramagnetic resonance (EPR) spectra of glass sample G-Mn-0.1 and glass-ceramic samples GC-1h and GC-5h measured at 85 K. |
Photoluminescence spectra of all samples are shown in Fig. 3a. Upon excitation at 250 nm, all doped glasses show a pronounced, broad emission band spanning the spectral range of 550 nm to 800 nm, originating from the spin-forbidden 4T1(4G) → 6A1(6S) transition in VIMn2+.30–32,41–44 With the increasing manganese content, the emission peak red-shifts gradually (Fig. 3a). At the same time, the emission intensity increases up to x = 2.0, and decreases for higher x, apparently due to concentration quenching. The PL decay curve follows-well a single exponential function for as long as x ≤ 0.5. At higher x, a multi-exponential function is required to fit the experimental decay data, indicating that at least two different emission species are active in these cases (Fig. 3c).
Fig. 3 (a) Emission spectra (λex = 250 nm) of as-prepared glass samples G-Mn-x, the values of Mn content x are indicated; (b) integrated emission intensities versus the Mn content x. Fitting to eqn (1) is performed with k = 9.1 ± 1.0, β = 1.8 ± 0.37, and θ = 6, depicted as the solid black line; (c) emission decay curve for glass samples G-Mn-x (λex = 250 nm, λem = 645 nm), the inset shows the dependence of lifetime on the Mn content x. (d) Excitation spectra (λem = 645 nm) of glass samples G-Mn-x, the inset shows the enlarged spectra in 400 to 550 nm. |
For a persistent luminescent material, the luminescence persistence is contributed by the activator lifetime, and the detrapping time which corresponds to the time when electrons are thermally discharged from the defect centers and then transferred to the activator, here Mn2+. To qualitatively understand how the PL lifetime changes with Mn2+ content, we define the PL lifetime as the time when the PL intensity decreases to the e−1 fold of the initial intensity. This was produced by fitting each curve to a single exponential equation and depicted as the inset of Fig. 3c. It is in the order of milliseconds for all samples and it implies the effect of concentration quenching. To reveal the mechanism which dominates the quenching effect, we fitted the concentration dependence of emission intensity to the classic equation:45
I = x × [k/(1 + β × (x/xc′)θ/3)], | (1) |
PL excitation spectra are shown in Fig. 3d. For all Mn2+-doped samples, they comprise a dominant band at ∼250 nm with a broad shoulder from 275 nm to 375 nm, and a well-separated sharp peak at ∼413 nm with another shoulder at approximately 500 nm. The corresponding band assignments are given in Fig. 3d.29–31 Also the excitation peaks exhibit a red-shift with increasing Mn2+ content. Obviously, the shoulder band peaking at about 500 nm extends to >550 nm. It therefore overlaps with the emission spectrum, meaning that re-absorption may occur especially at higher content of Mn2+ (see Fig. 3a and d). This is taken as one of the reasons for the observed red-shift in the emission and excitation spectra. Noteworthy, there is another weak band occurring at around 410 nm. We assign this to the presence of oxygen-defects on Ge4+ entities, Ge-associated oxygen defects (GODCs).34,46 Similar defects have been observed in germanate glasses previously.31,46 Their intensity decreases with increasing Mn2+ content due to energy transfer from GODCs to Mn2+.
Fig. 6 TL curves of glass sample G-Mn-0.1 and glass-ceramic samples GC-0.5h, GC-1h, GC-2h and GC-5h as a function of temperature. |
Samples | Peak1 | Trap1 | Peak 2 | Trap2 | Peak 3 | Trap3 |
---|---|---|---|---|---|---|
T m (K) | E (eV) | T m (K) | E (eV) | T m (K) | E (eV) | |
G-Mn-0.1 | 354 | 0.71 | 401 | 0.80 | 529 | 1.06 |
GC-0.5h | 348 | 0.70 | 376 | 0.75 | 416 | 0.83 |
GC-1h | 349 | 0.70 | 374 | 0.775 | 417 | 0.83 |
GC-2h | 347 | 0.69 | 372 | 0.74 | 417 | 0.83 |
GC-5h | 348 | 0.70 | 376 | 0.75 | 423 | 0.85 |
With the removal of the excitation source, all glass ceramics exhibit a more intense and longer afterglow. This is especially the case for sample GC-5h (see Fig. 8). Noteworthy, even after 104 h, some afterglow could still be detected. Fig. 9 shows the time resolved emission spectra of GC-5h, which were measured at different delay times from 15 seconds to 104 hours. A strong blue-shift of the emission peak occurs within the first 15 s, i.e., from 671 nm to 635 nm (see Fig. 9 and 7a). Subsequently, the peak slightly red-shifts to ∼650 nm (see the 30 h to 104 h delayed curves in Fig. 9). This implies multiple VIMn2+ emission centers in the glass ceramic sample. The green PL band which was attributed to IVMn2+ does not show persistent luminescence after removal of the excitation source (see Fig. 9).
Fig. 8 Afterglow decay curves of glass sample G-Mn-0.1 and glass-ceramic samples GC-0.5h, GC-1h, GC-2h and GC-5h. |
TL, XRD and EPR spectra of glass ceramic samples were collected to understand the observed differences between glass and glass ceramic samples. The TL spectra indicate that the strongest peak occurs at ∼360 K in the glass ceramics, at a lower temperature than in the glassy specimen (see Fig. 6). This means that the energetic depth of the underlying defect is slightly lower. Again, we obtain an accurate fit with three Gaussian bands (Table 1). For GC-5h, the corresponding defect depths are 0.70 eV, 0.75 eV and 0.85 eV, respectively, all lower than the ones observed in G-Mn-0.1. This might be due to the substitution of gallium ion with a higher ionization energy (IE) 2963 kJ mol−1 for the aluminum ion (IE = 2745 kJ mol−1) in glass ceramic samples as will be discussed below. The decrease of the content of the codopant with lower IE will make the traps shallower. This was observed similarly in ZnS:Cu+,Co2+.14 The much higher intensity of TL emission further indicates a much higher number density of those defects.
Fig. 10 shows the XRD pattern of the glass samples G-Mn-0.1 after different crystallization times (0–5 h). As expected, there are no obvious diffraction peaks in the glass sample, nor in the GC-0.5h sample. This indicates that those two samples are amorphous, or that the crystals are too small to cause lattice diffraction. For GC-0.5h, we expect the latter because we did observe the green emission band in the PL spectra which we took as evidence for the occurrence of some IVMn2+. In GC-1h, GC-2h and GC-5h, sharp diffraction peaks appear and grow further with prolonged treatment time. We use Scherrer's equation to obtain a rough estimate of the corresponding crystallite size, D = Kλ/βcosθ, where D is the crystallite size, K is a dimensionless shape factor, λ is the X-ray wavelength, β (rad) is the line broadening at half maximum (FWHM), and θ (rad) is the Bragg angle. The strongest diffraction peak with 2θ = 27.02° was selected for the calculation, yielding an average size of ∼30 nm, 50 nm and 60 nm for GC-1h, GC-2h and GC-5h, respectively. For GC-5h, the morphology and size distribution of those nanocrystals was also analyzed by HR-TEM, see Fig. 11a, where irregular crystallites with size of ∼20–50 nm were observed. The lattice fringes can be observed in a higher resolution image, as the inset of Fig. 11a shows, and the distance between neighboring crystal lattice fringes is ∼0.328 nm, and it corresponds to the (002) crystal facet of albite Na(Al,Ga)Ge3O8. This consists of the diffraction patterns of Fig. 10, which point to a triclinic phase of space group C, NaAlGe3O8 (ICSD #61170). Ga3+ can readily substitute for Al3+ to form the gallium albite, Na(Al,Ga)Ge3O8, which we expect to occur in the present case. We relate the presence of Al3+ to crucible dissolution, which was confirmed by two-dimensional element mapping analysis as shown in Fig. 11b–f. Sodium, germanium, aluminum, gallium and oxygen are homogeneously distributed throughout the sample.
The Rietveld refining was made with FullProf Suite Program version 2011, and it starts with the crystallographic data of NaAlGe3O8 (ICSD #61170). The refining produces lattice parameters of a = 8.4063 Å, b = 13.2844 Å, c = 7.3389 Å, α = 94.39, β = 116.28, γ = 90.86 and V = 731.59 Å3 for sample GC-5h. The cell volume is a little bigger than 731.47 Å3 (ICSD #61170). This expansion of the cell is a sign for the incorporation of gallium into the NaAlGe3O8 compound and, thus, for the formation of the solid solution Na(Al,Ga)Ge3O8. It is also consistent with the shifts of the diffraction peaks to somewhat lower angles as compared to the pure high-albite (see Fig. 10). High-albite comprises one type of octahedral sodium sites and four types of tetrahedral Ge/Al/Ga sites. When manganese ions are introduced, considering their radius alone, they can assumedly substitute for either sodium or for any of the tetrahedral sites. The latter explains the occurrence of some green PL, while the former is taken to be at the origin of prolonged afterglow.
Fig. 12 Schematic of the mechanism of persistent luminescence processes in the Mn2+ doped glasses and glass-ceramics. EC1, EC2 and EC3 stand for the different types of octahedral Mn2+ emission centers; for clarity, only the ground state 6A1 (6S) and the first excited state 4T1 (4G) of Mn2+ are shown. D1, D2 and D3 stand for the different types of defects with depth as shown in Table 1. Dark red solid circles are electrons while white solid circles are holes. Paths 1 to 6 denote the different processes involved in the generation of the persistent luminescence processes. |
When the samples were illuminated with ultraviolet light, different types of Mn2+ emission centers (named EC1, EC2 and EC3 but the number is for sake of visualization in Fig. 11) in the hosts are excited along path 1 into the 4T1 (4P) state, the latter lying in the conduction band. This leads to a separation of electrons and holes. Holes subsequently follow path 2 and flow into the valence band. Simultaneous removal of the electron and hole will not lead to the valence variation of the manganese ion. Some electrons, on the other side, relax to the lower end of the conduction band via path 3. Another portion of electrons transits through the non-radiative path 4 to the different types of the shallow defects such as D1, D2 and D3. D1–D3 have the depths as summarized in Table 1. When the excitation source is removed, electrons start to escape from the traps D1–D3 via path 5. Path 5 is thermally activated and it, hence, is slow at room temperature. The electrons can also be removed from D1 (or D2, D3) directly, and be trapped on the excited states of EC1 (or EC2, EC3). They can further also escape from a deeper defect to a shallower defect reversibly, and then be captured by the activators. In the meanwhile, thermal stimulation will lift holes from the valence band to the ground state via path 2. Once the electrons are trapped on EC1 (EC2, EC3), they emit their potential energy in the form of red light by recombining to holes. The relaxation dynamics, in turn, depend on the depth and the density of defects D1 to D3. As the Mn2+ doped glass is transformed into the glass ceramic, the defects become shallower and more populated. We did try to charge these shallow defects with other light sources such as 365 nm or 410 nm LEDs, but failed. This confirms that path 1 is the crucial process for lifting the electrons into the conduction band directly. Once electrons are in the conduction band, they can move freely and, especially, charge the defects. The defects, as discussed in ref. 14, may not be randomly distributed throughout the sample. They with depth as presented in Table 1 prefer gathering in the vicinity of manganese sites. It, therefore, enables energy transfer possible from the defects to the activators.
In view of the ultralong lasting afterglow, we had tried to synthesize Mn2+ doped NaAlGe3O8 crystals by traditional high temperature solid state reaction but failed. Further studies are obviously necessary on how to prepare the high pressure phase with a controlled size and morphology by other techniques rather than the current technique of crystallization from the glass phase. Usually for nanosized persistent luminescence materials, the persistence will be greatly shortened especially as compared to bulk materials. This work, however, differently, shows that the persistence can be preserved longer than 100 h even when the doped crystalline phase of Na(Al,Ga)Ge3O8 becomes as small as 50 nm. This, plus luminescence in the 600 nm to 800 nm range lying in the tissue transparent region, demonstrates its potential application in tumor bioimaging or photodynamic therapy. This study is the first and primary step towards it.
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