Nilesh B. Shelkea,
Ramrao Ghorpadea,
Ajay Pratapa,
Vijay Takb and
B. N. Acharya*a
aProcess Technology Development Division, Defence R & D Establishment, Jhansi Road, Gwalior-474002, MP, India. E-mail: bnacharya@drde.drdo.in; bnacharya@yahoo.com
bVertox Laboratory, Defence R & D Establishment, Jhansi Road, Gwalior-474002, MP, India
First published on 26th March 2015
N-Arylation of amines with fluorobenzonitriles in aqueous medium is described. A mixture of N,N-diisopropylethyl amine and Na2CO3 (1:
1) is found to achieve maximum conversion by refluxing for 3 hours in water. The product can be easily isolated by solvent extraction.
During the course of our ongoing research activity on bioactive compounds, we were exploring suitable protocols to synthesize 2,4-diaminoquinazolines. Quinazolines and quinazolones are bioactive molecules also part of various natural products and drugs.7 Among the quinazolines, 2,4-diaminoquinazolines are very important pharmacophore present in many marketed drugs such as prazosin,8a methotrexate,8b trimetrexate,8c and piritrexim.8d They have shown significant biological activities such as anticancer,9a SMN2 promoter,9b dihydrofolate reductase (DHFR) inhibitor,9c kinase inhibitor and opioid receptor like-1 (ORL1) antagonists.9d
Traditionally, 2,4-diaminoquinazolines are synthesized by reacting 2-fluorobenzonitrile with guanidine carbonate at elevated temperature via aromatic SNAr reaction.10 Alternatively, they can also be synthesized by Cu-catalyzed Ullmann type N-arylation of 2-bromobenzonitrile with guanidine.11
The reaction was optimized using 2-fluorobenzonitrile as substrate and guanidine carbonate as nucleophile. The first reaction was carried out with reference to a reported method in N,N-dimethylacetamide (DMA) at reflux condition with moderate yield12 (Table 1, entry a). With the introduction of a base, the yield of the reaction improved significantly (entry b). However this reaction was not feasible at lower temperature (entry c). Several inorganic and organic bases were tried at elevated temperature from which Na2CO3 and diisopropylethylamine (DIPEA) were found to give good yield. Reaction in presence of DIPEA was carried out at 125 °C. The yield was found slightly less than the same reaction carried out in presence of Na2CO3 (entry l). Then we thought to use a mixture of Na2CO3 and DIPEA in order to see the effect on yield (entry m to q). A very significant improvement in yield was observed when both the bases were used in 1:
1 mole ratio (entry o). With this encouraging result, we tried to explore the greener aspect of this reaction by replacing DMA with water. To our disappointment the reaction didn't proceed even at reflux condition (entry r). The reason may be attributed to the very high water solubility of guanidine carbonate and poor water solubility of 2-fluorobenzonitrile. This solubility difference may keep these two reactants in immiscible phases due to which the reaction didn't occur at all even in presence of tetrabutylammoniumbromide (TBAB), a phase transfer catalyst (PTC).
Reactn code | Inorganic base (mol%) | Organic base (mol%) | Temp. (°C) | Time (hrs) | Yieldb |
---|---|---|---|---|---|
a Reaction conditions: 2-fluorobenzonitrile (1 mmol), guanidine carbonate (1 mmol), base (2 equiv.; organic/inorganic), DMA (3 mL), reaction time 3 h. DMA: N,N-dimethylacetamide, DIPEA: diisopropylethylamine, DBU: 1,8-diazabicyclo[5.4.0]undec-8-ene, DBN: 1,5-diazabicyclo[4.3.0]non-5-ene, TEA: triethylamine.b Isolated yield.c Reaction medium: water. | |||||
a | 0 | 140 | 8 | 37 | |
b | Na2CO3 (100) | 140 | 8 | 72 | |
c | Na2CO3 (100) | 60 | 8 | 0 | |
d | K2CO3 (100) | 140 | 8 | 25 | |
e | NaHCO3 (100) | 140 | 8 | 55 | |
f | DBU (100) | 60 | 8 | 0 | |
g | DBU (100) | 100 | 8 | 0 | |
h | DBU (100) | 140 | 8 | 31 | |
i | DBN (100) | 140 | 8 | 22 | |
j | TEA (100) | 125 | 8 | 0 | |
k | DIPEA (100) | 125 | 8 | 63 | |
l | Na2CO3 (100) | 125 | 8 | 10 | |
m | Na2CO3 (90) | DIPEA (10) | 125 | 3 | 45 |
n | Na2CO3 (70) | DIPEA (30) | 125 | 3 | 48 |
o | Na2CO3 (50) | DIPEA (50) | 125 | 3 | 79 |
p | Na2CO3 (30) | DIPEA (70) | 125 | 3 | 63 |
q | Na2CO3 (10) | DIPEA (90) | 125 | 3 | 55 |
rc | Na2CO3 (50) | DIPEA (50) | 100 | 8 | 0 |
To check the feasibility of above protocol for SNAr, a batch reaction was carried out in water using 2-fluorobenzonitrile as substrate and piperidine as nucleophile in place of guanidine carbonate. Around 40% yield was observed (Table 2, entry a). When the same reaction was carried out in DMA at 100 °C surprisingly 88% yield was recorded (entry b). Choices of solvent and base are crucial for catalyst free SNAr reactions. Both the reactants are insoluble in water while both Na2CO3 and DIPEA are highly water soluble. Probably they are not available in the reaction due to phase separation. All the reactants and bases are soluble in DMA which is leading for a very good conversion. With the introduction of TBAB, a significant improvement in yield was observed in water (entry c and d). Couple of reactions were carried out with reference to a reported method in DMA by using Cs2CO3 as base.2a Moderate yield of product was observed (entry e). As the reaction was carried out in a conventional laboratory assembled reactor, some part of piperidine (bp 106 °C) might have been lost to give moderate yield (entry e). At lower temperature the yield was found even lower (entry f). Most interestingly yield was reduced, when the reaction was carried out in absence of either inorganic (entry g) or organic base (entry h). This indicated that both organic and inorganic bases are required whether the reaction was carried out either in DMA or in water. No conversion was observed when the reaction was carried out at room temperature (entry i). Organic bases like DBU, DBN, and TEA also showed promising yields (entries j and k) but DIPEA gave maximum yield among them.
Entry | Solvent | Temp. (°C) | Organicbase | Inorganicbase | TBAB (mol%) | Yieldb [%] |
---|---|---|---|---|---|---|
a Reaction conditions: 2-fluorobenzonitrile (1 mmol), piperidine (1 mmol), base (2 equiv.; organic/inorganic 1![]() ![]() |
||||||
a | Water | 100 | DIPEA | Na2CO3 | 0 | 40 |
b | DMA | 100 | DIPEA | Na2CO3 | 0 | 88 |
c | Water | 100 | DIPEA | Na2CO3 | 5 | 66 |
d | Water | 100 | DIPEA | Na2CO3 | 10 | 83 |
e | DMA | 140 | Cs2CO3 | 0 | 61 | |
f | DMA | 100 | Cs2CO3 | 0 | 35 | |
g | Water | 100 | DIPEA | 10 | 40 | |
h | Water | 100 | Na2CO3 | 10 | 30 | |
i | Water | 30 | DIPEA | Na2CO3 | 10 | 0 |
j | Water | 100 | DBU | Na2CO3 | 10 | 65 |
k | Water | 100 | DBN | Na2CO3 | 10 | 58 |
l | Water | 100 | TEA | Na2CO3 | 10 | 71 |
From these optimization experiments we have standardized two protocols for the synthesis of 2,4-diaminoquinazolines (protocol 1, Table 3) and derivatization of fluorobenzonitriles (protocol 2, Table 4). The latter one can be carried out in both DMA and water. To study the general applicability of the present developed methods, the standardized reaction conditions were attempted with different halobenzonitriles with various amines and are presented in Tables 3 and 4 respectively. As shown in the Table 3, the desired 2,4-diaminoquinazolines were obtained in good to excellent yields (entries a–e). The protocol is not found effective for 2-chlorobenzonitrile (entry g).
Entry | ArX (1) | Product (3) | Yieldb |
---|---|---|---|
a Protocol 1: 2-fluorobenzonitrile (1 mmol), guanidine carbonate (1 mmol), base (2 equiv.; DIPEA/Na2CO3 1![]() ![]() |
|||
a | ![]() |
![]() |
79 |
b | ![]() |
![]() |
88 |
c | ![]() |
![]() |
52 |
d | ![]() |
![]() |
48 |
e | ![]() |
![]() |
25 |
f | ![]() |
![]() |
44 |
g | ![]() |
![]() |
19 |
Entry | ArX (1) | Amine (2) | Time | Yieldb |
---|---|---|---|---|
a Protocol 2: 2-fluorobenzonitrile (1 mmol), piperidine (1 mmol), base (2 equiv.; DIPEA/Na2CO3 1![]() ![]() |
||||
a | ![]() |
![]() |
3 | 83 |
b | ![]() |
(2a) | 3 | 87 |
c | ![]() |
(2a) | 3 | 82 |
d | ![]() |
(2a) | 3 | 76 |
e | ![]() |
(2a) | 3 | 81 |
f | ![]() |
(2a) | 3 | 79 |
g | ![]() |
(2a) | 8 | 75 |
h | (1a) | ![]() |
3 | 85 |
i | (1b) | ![]() |
5 | 79 |
j | (1b) | ![]() |
3 | 72 |
Protocol 2 was carried out in water to check the feasibility of the green aspect. Various substrates including fluorobenzonitriles, dinitrochlorobenzene. Excellent yields were recorded in all cases (Table 4, entries a–e). Incase of fluoroquinazolines no conversion was observed.
Excellent yield for 2,4-dinitrochlorobenzene was also observed (entry f). 2,5-Diflurobenzonitrile (entry g) showed good conversion only after extended reaction time. This shows presence of electron withdrawing groups at 2 and 4 position are beneficial where as at 5 position it is not beneficial for product yield. This indicates that the reaction mechanism is following SNAr pathway. Other nitrogen containing nucleophiles also witnessed good yield with this protocol (entries h to j). The products were isolated by simple solvent extraction with ethylacetate and purified by flash chromatography.
The most important finding of this study is the effect of the mixture of organic and inorganic bases on yield. A plausible mechanism is attempted in Scheme 1 on the basis of experimental observations, semiempirical calculations and literature report.13
2-Fluorobenzonitrile and piperidine react with each other to form a transition state (TS1) in the organic phase. TBAB helps the transport of amine (piperidine) as well as DIPEA from aqueous phase to organic phase and thus plays a significant role to enhance the rate of reaction. TS1 converts to an intermediate (IM) after migration of hydrogen from amine to nitrile group. At the same time the organic base DIPEA forms a strong hydrogen bonding with the intermediate and forms another transition state (TS2). The energy of transition state in presence and absence of DIPEA was calculated by semiempirical method AM1 (Fig. 1).14 Transition state 2 (TS2) and IM are stabilized by DIPEA. The difference in transition state energy of TS2 clearly indicates the involvement of DIPEA for facilitation of the reaction. This was also reflected in the % yield of product (Table 2, entry d and entry h). As fluorine is a better leaving group in SNAr reaction, it leaves the ring with pair of electron. Simultaneously base abstracts the hydrogen and a pair of electron is available to satisfy aromaticity. The product remains in the organic phase and the fluoride anion is sequestered out from the organic phase to aqueous phase by the cation of DIPEA. The organic base was regenerated in aqueous phase by the inorganic base Na2CO3 and again comes to organic phase. Thus the mixture of organic and inorganic bases enhances the yield through above mechanism.
![]() | ||
Fig. 1 Relative energy of starting material, transition states and intermediates with respect to products in presence (solid line) and absence (dotted line) of DIPEA. |
From this study we may conclude that a mixed organic and inorganic base system is useful to achieve better yield in N-arylation of amine through SNAr mechanism. The reaction may be carried out in aqueous medium. The inorganic base will remain in the aqueous phase to regenerate the organic base due to which the yield will increase.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c5ra03510d |
This journal is © The Royal Society of Chemistry 2015 |