Arnulfo
Rojas-Pérez
a,
Daysi
Diaz-Diestra
ab,
Cecilia B.
Frias-Flores
a,
Juan
Beltran-Huarac
ab,
K. C.
Das
c,
Brad R.
Weiner
ab,
Gerardo
Morell
ab and
Liz M.
Díaz-Vázquez
*a
aDepartment of Environmental Sciences, Physics and Chemistry, University of Puerto Rico, San Juan, PR 00936, USA. E-mail: lizvazquez8@gmail.com
bMolecular Science Research Building, University of Puerto Rico, San Juan, PR 00926, USA
cBiorefining and Carbon Cycling Program, Department of Biological and Agricultural Engineering, University of Georgia, Athens, GA 30602, USA
First published on 5th October 2015
We report a comprehensive quantitative study of the production of refined bio-crudes via a controlled hydrothermal liquefaction (HTL) process using Ulva fasciata macroalgae (UFMA) as biomass and ultrananocrystalline Fe3O4 (UNCFO) as catalyst. X-ray diffraction and electron microscopy were applied to elucidate the formation of the high-quality nanocatalysts. Gas chromatography-mass spectroscopy (GC-MS) and CHNS analyses showed that the bio-crude yield and carbon/oxygen ratios increase as the amount of UNCFO increases, reaching a peak value of 32% at 1.25 wt% (a 9% increase when compared to the catalyst-free yield). The bio-crude is mainly composed of fatty acids, alcohols, ketones, phenol and benzene derivatives, and hydrocarbons. Their relative abundance changes as a function of catalyst concentration. FTIR spectroscopy and vibrating sample magnetometry revealed that the as-produced bio-crudes are free of iron species, which accumulate in the generated bio-chars. Our findings also indicate that the energy recovery values via the HTL process are sensitive to the catalyst loading, with a threshold loading of 1.25 wt%. GC-MS studies show that the UNCFO not only influences the chemical nature of the resulting bio-crudes and bio-chars, but also the amount of fixed carbons in the solid residues. The detailed molecular characterization of the bio-crudes and bio-chars catalyzed by UNCFO represents the first systematic study reported using UFMA. This study brings forth new avenues to advance the highly-pure bio-crude production employing active, heterogeneous catalyst materials that are recoverable and recyclable for continuous thermochemical reactions.
Recently, the production of bio-crude employing macroalgae as a potential biomass source has been considered due to their high energy content, low life-cycle emission of greenhouse gases, relatively fast growth rates, ease of harvesting and cost-effectiveness.6 For instance, they possess higher lipid contents than most lignocellulosic biomass (such as plants and wood) used for biofuels, and are primarily composed of alginic acid, laminarin, carrageenan and agarose, which are critical to facilitate thermochemical conversion processes.7 Although the HTL process has the potential to generate high yields of bio-crude, there are some limitations to using macroalgae biomass as a feedstock. In addition, macroalgae exhibit high ash content (up to 50%) due to the presence of inorganic salts and metals, which restricts their use in direct combustion and gasification processes. Few studies have reported the effect of ash content in HTL conversion of marine feedstock (such as macroalgae).8 The high inorganic content in macroalgae would affect the physicochemical characteristics of the bio-crude and its storage through the catalysis of polymerization reactions. Neveux et al.3 compared the bio-crude yield of six macroalgae using a stainless steel reactor covering a temperature range of 330–341 °C and pressures between 140–170 bar, finding that Ulva ohnoi exhibits a high crude yield of ∼18.7 wt%. Singh et al.4 evaluated the use of ethanol, methanol and water as solvents in the liquefaction of Ulva fasciata. The total crude yield obtained was 11 wt% for water, 40 wt% for ethanol, and 44 wt% for methanol, showing that alcohols increase the bio-crude yield acting not only as co-solvents but also as hydrogen donors that promote further transesterification reactions.
More recently, efforts have been devoted to investigating the effect of catalysts on the HTL process and its correlated efficient path to produce high-quality bio-crude.5,9 Among the most used catalysts, solid nanocatalysts have drawn special attention due to their high chemical and physical stability and larger chemically-active surface areas, both being critical factors in industrial applications.10,11 Nonetheless, the recovery of the solid catalysts from the bio-crude mixture via conventional filtration process is generally not cost-effective. For this reason, it is important to develop heterogeneous catalyst materials that are recoverable and recyclable for continuous thermochemical reactions. In this context, iron oxide-based nanocatalysts have been proposed as an alternative because they can be effectively separated by means of magnetic fields,12 thus enhancing their lifetime and cost-effectiveness. Moreover, iron oxide nanostructures possess large specific surfaces, low mass transfer resistance, and improved catalytic activity for photocatalysis, biocatalysis, and phase-transfer catalysis.13 Few studies on the bio-diesel production using iron oxide-based nanocatalysts through transesterification have been reported to date. Ying et al.14 used a magnetic cell biocatalyst immobilized in hydrophobic magnetic polymicrosphere for the transesterification of waste cooking oils with methanol, obtaining a conversion up to 90%. Xie et al.15 immobilized lipase on Fe3O4 nanoparticles (NPs) as a catalyst for biodiesel production by transesterification of soybean oil with assisted-methanol, and achieved a conversion over 90%. Hu et al.16 prepared a magnetic porous nanocatalyst based on KF/CaO–Fe3O4 and obtained a fatty acid methyl esters yield of 95%. Hence, iron oxide-based nanocatalysts exhibit high catalytic activity and significant advantages for easy magnetic separation and reusability. However, this research field is still at its infancy and further investigation on the bio-crude yield is needed. Furthermore, the bio-crude production via HTL assisted by Fe3O4 catalysts using marine products as feedstock remains a big challenge.
In this study, we report for the first time the quantitative effect of ultrananocrystalline Fe3O4 (UNCFO) catalyst on the production of refined bio-crude via a controlled HTL process using Ulva fasciata macroalgae (UFMA) as biomass. A comprehensive analysis of the molecular characterization of the bio-crudes is presented. The ability of the UNCFO catalyst to influence the chemical composition of the resulting bio-crudes and the amount of fixed carbon in the solid residues is also discussed.
![]() | (1) |
HHV (MJ kg−1) = 0.3383C + 1.422(H–O/8) | (2) |
![]() | (3) |
The catalytic capability and efficiency of the as-synthesized UNCFO to produce refined bio-crudes were tested via CHNS/O analysis. Table 1 shows the yield, higher heating values (HHV), and energy recovery (ER) of the bio-crude and bio-char after HTL processing of UFMA employing different UNCFO loadings and the reaction conditions proposed by Singh et al.4 on the same biomass. These conditions are in the maximal bio-crude yield range reported by Shi et al.24 Guo et al.25 and many other studies.26–32 It was found that the baseline (i.e., no catalyst) bio-crude yield is 23.3 ± 0.4% for the dry biomass in ash-free basis, which was higher than the value reported (11%) by Singh and coworkers,4 even when adjusted to our applied formula (18.78%). The increase on the yield could be due to the dichloromethane extraction in our separation process, i.e., a larger range of compounds can be extracted from the liquid mixture instead of the exclusive high non polar molecules extracted with diethyl ether in their experiments. All other reaction conditions were kept constant, even the proximate analysis performed over the biomass (Table 2) showed similar UFMA composition.
UNCFOs (wt%) | Bio-crude Yield (%) | Bio-crude HHV (MJ kg−1) | Bio-crude ER (%) | Bio char (%) | Bio char HHV (MJ kg−1) | Bio-crude ER (%) | Gas yield (%) |
---|---|---|---|---|---|---|---|
a ER: energy recovery and HHV: higher heating value. | |||||||
0 | 23.30 ± 0.42 | 32.39 ± 0.24 | 56.52 ± 0.8 | 11.90 ± 0.01 | 30.26 ± 1.81 | 18.78 ± 1.03 | 37.06 ± 0.53 |
0.63 | 31.17 ± 0.53 | 29.64 ± 1.48 | 69.20 ± 1.2 | 12.52 ± 0.07 | 24.19 ± 0.14 | 28.67 ± 1.01 | 35.55 ± 0.22 |
1.25 | 32.36 ± 0.42 | 30.71 ± 0.66 | 74.42 ± 0.7 | 13.22 ± 0.02 | 17.62 ± 3.09 | 21.92 ± 0.98 | 32.10 ± 0.37 |
2.50 | 25.99 ± 2.11 | 30.80 ± 1.50 | 59.92 ± 1.4 | 13.33 ± 0.04 | 13.46 ± 0.10 | 18.02 ± 1.11 | 36.50 ± 0.92 |
3.75 | 25.48 ± 1.08 | 31.90 ± 0.56 | 60.87 ± 1.0 | 13.95 ± 0.04 | 11.27 ± 3.92 | 15.73 ± 1.12 | 44.91 ± 1.13 |
Likewise, an enhanced bio-crude production of 32.3 ± 0.4% (a ∼9% of increase when compared to the catalyst-free yield) is obtained when 1.25 wt% of UNCFO is added. This finding is not directly comparable with the literature because there are very few studies on HTL of Ulva species and no one employed heterogeneous nanocatalyst.3,4,33 However, it is possible to find a similar increasing trend in the Duan & Savage work for the catalytic bio-crude production of Nannochloropsis sp. by using bulk commercial catalysts (Pd/C, Pt/C and Ru/C).5 The best UNCFO run shows the same proportional increase between the non-catalyzed and catalyzed reaction for noble metals. Although the metal catalyst function and catalytic mechanism are not clearly understood24 it is necessary to mention that the catalyst loading in the mention work was 50 wt% with respect to dry biomass, an excessive amount of catalyst when compared with our NPs loading. Jena and coworkers studied the roll of bulk NiO in the catalytic HTL of the microalgae Spirulina platensis26 and it was found that the amount of oil decreased up to 24.3% when compared with non-catalytic runs while its HHV and gas production increased. Also by using Ni as a hydrothermal liquefaction catalyst, a significant increase of C in the gas phase was found. The authors argued that the Ni presence lead to tertiary reactions of organics in the bio-crude that increase the production of gases thus provoking a decrease in the bio-oil yield. All the data reported suggested that the bio-crude yield and HHV are affected by the amount and type of catalyst added.
The HHV of our bio-crude remained essentially constant between 30–32 MJ Kg−1. As for the ER of the bio-crude, the highest ER value found was 74.4 ± 0.7% for a UNCFO loading of 1.25 wt%, which represents a ∼18% of increase in absence of the catalyst. This observation is compatible with the corresponding increments of the yield and HHV after the HTL process. Similarly, the drop of ER values at 2.50 and 3.75 wt% correlate well with the decrease of their corresponding yields and show that the catalyst loading has a nonlinear effect on the conversion and yield. Similar behaviors have been reported in the literature for the use of iron-based catalysts in thermochemical conversion reactions.34–36 For example, Dadyburjor and coworkers reported a nonlinear correlation between ferric sulfide catalyst loading and the yield of oil in the coal liquefaction.34 Bacaud and coworkers studied different iron-based catalysts for the liquefaction of coal and reported that optimal results are obtained at low ratios of catalyst/coal 0.2 to 2% iron oxide and red mud.36 The nonlinear correlation between the loading of the catalyst and the bio-crude yield, may be attributed to different factors or a combination of them: (i) at higher loading of the catalyst, the probability of particle agglomeration increases, thus reducing the surface-to-volume ratio for interaction with the catalyst; (ii) the catalyst enhances hydrogenation, but at higher loadings H2 is mainly consumed in the production of gases, as shown in Table 1; (iii) another possibility is that the catalyst may mediate retrograde reactions in which some products combine to produce heavier compounds that are not recovered in the bio-crude phase. Table 1 summarizes the detailed list of the values obtained for the yield, HHV and ER of the bio-char and bio-crude of marine UFMA. Taken altogether, it is clear that the HTL process is sensitive to the catalyst loading, reaching the highest value at 1.25 wt%, and that the catalyst substantially influences the chemical nature of the resultant bio-crude. In order to shed more light upon the chemical compositions of the bio-crudes, we employed FTIR spectroscopy.
The FTIR spectra of the bio-crude at different UNCFO loadings are presented in Fig. 2a, wherein the FTIR spectrum of the raw algae was included for comparison. As a whole, the spectra exhibit similar vibrational modes in the presence of the catalyst. The bands peaking at 723, 744 and 883 cm−1 indicate the presence of aromatic compounds. The vibrations observed can be classified, as follows: (i) the O–H bending characteristic of phenols, esters and ethers centered at 1039 cm−1, (ii) the C–O stretching characteristic of alcohols peaking at 1145 and 1288 cm−1; (iii) the C–H bending characteristic of fats at 1380 and 1458 cm−1, (iv) the CC stretching characteristic of alkenes centered at 1612 cm−1, (v) the C
O stretching characteristic of carboxylic acids, ketones and aldehydes detected at 1672 and 1697 cm−1, (vi) the C–H stretching characteristic of alkyl groups peaking at 2869, 2927 and 2933 cm−1, and (vii) the water traces centered at 3350 cm−1. The difference in intensity of the bands was attributed to the relative degree of abundance of certain compounds. Although the diversity of the functional groups present in the bio-crudes is apparently complex, the catalytic effect of UNCFO on the bio-crudes is marked in terms of the amount of fixed carbons that can be generated in the solid residues. Similar FTIR profiles were previously reported by other research groups working with Ulva prolifera via microwave-assisted direct liquefaction, and with marine brown alga via HTL.21,33 No traces of iron species were detected in the bio-crudes at all UNCFO loadings, as expected. To further corroborated this observation, we conducted vibrating sample magnetometry given that UNCFO is magnetized when an external magnetic field is applied. The magnetic hysteresis loops of pristine UNCFO and of a representative bio-crude are displayed in Fig. 2b. It was observed that the pristine UNCFO reaches the magnetization saturation at ∼15 kOe, and shows a coercive field (HC) of 16.1 Oe with a near-zero remnant field, indicating a well-defined superparamagnetic behavior.23 No magnetic response was observed in the bio-crude, confirming the absence of iron species therein.
![]() | ||
Fig. 2 (a) FTIR spectra of raw UFMA and bio-crudes produced using different UNCFO loadings, and (b) M–H hysteresis curves of pristine UNCFO and a representative bio-crude. |
As described above, the bio-crudes generated from the HTL process of UFMA in the presence and absence of the nanocatalyst are complex mixtures composed of more than 100 compounds; thus their identification is somewhat cumbersome. In order to obtain a more detailed characterization of the compounds, we conducted GCMS analysis. The chromatograms were analyzed using the NIST library and only compounds having a quality identification factor higher than 70 are reported. Fig. 3 shows: (a) the distribution of bio-crude compounds categorized by their energy related functionalities, (b) the most abundant fatty acids, and (c) the most abundant nitrogen containing species. It was observed that the bio-crudes are mainly composed of fatty acids, esters, alcohols, nitrogen containing heterocyclic species, hydrocarbons, aldehydes, phenols, benzene derivatives, aminoacids, aromatic compounds and ketones. The detection of these compounds is consistent with the FTIR analysis. We have identified five main groups of compounds (fatty acids, alcohols, ketones, phenols and benzene derivatives, and hydrocarbons) with highly energetic functionalities in the bio-crudes, as shown in Fig. 3a. It was also found that the nitrogen containing heterocyclic compounds experience the most prominent change. In general, the variety and concentration of this type of compounds increased as the amount of catalyst increased from 0.63 to 2.50 wt% (see Fig. 3c). The presence of nitrogenated compounds (including the aromatic heterocyclic organic compound, indole) is associated to the decomposition of algal proteins. In parallel, a higher abundance of pyrazine, pyridines and other related derivatives in the presence of the catalyst was also identified, which is ascribed to the reactions between algal reducing sugars and amino acids (Maillard's reaction).37 This is indicative of UNCFO's strong catalytic ability to favor the formation of specific products in Maillard's reactions, and is attributed to their high redox potential.39 In terms of fatty acids, the variety of this family of compounds decreased in the presence of the catalyst (see Fig. 3a). In contrast, the abundance of propanoic acid (a short-chain fatty acid) increased in the presence of the catalyst, as seen in Fig. 3b. It was also observed that the abundance of arachidonic and tetradecanoic acids (long-chain fatty acids) do not show a well-defined tendency with respect to the UNCFO loadings, except the hexadecanoic acid that increases its abundance as the amount of catalyst increases. This indicates that the bio-crudes with the highest values of HHV contain higher abundances of long-chain fatty acids (C14–C20). Further observations also show the formation of additional derivatives, such as 9-methyl-tetradecanoic acid pyrrolidide, when UNCFO is used, which are associated to the condensation reaction between the fatty acids and the products obtained from the thermal decomposition of algal proteins. Their origin may be attributed to the interaction that fatty acid compounds generate with the charged surface of UNCFO, which provides an anchoring spot that catalyzes the condensation of diverse compounds with more complex structures.38 This is supported by the fact that most metal oxides exhibit an effective influence on the chemical nature and amount of the resultant humic products (including supernatant and solid residues), which are governed by Maillard's reactions.37 Accordingly, UNCFO substantially influences the chemical nature of the resulting bio-crudes and the amount of carbons fixed in the solid residues, in agreement with the results detailed in Table 3. Our observations also reveal that the alcohols (including butanol, hexanol and cyclohexanol) present in the bio-crudes show a tendency similar to that found for nitrogenated compounds.
![]() | ||
Fig. 3 Distribution of bio-crude compounds categorized by their (a) energy related functionalities, and content of (b) common fatty acids present in algae and (c) nitrogen containing species. |
UNCFO (wt%) | C (%) | H (%) | N (%) | S (%) | Oa (%) | C/O ratio | |
---|---|---|---|---|---|---|---|
a Obtained by difference, N/A: not applicable, N.D: below detection limits or not detected. | |||||||
Raw U. fasciata | N/A | 26.29 ± 2.96 | 4.60 ± 0.31 | 2.66 ± 0.13 | 3.13 ± 0.61 | 49.05 ± 3.83 | 0.60 ± 0.14 |
Bio-crude | 0 | 70.35 ± 1.42 | 8.00 ± 0.35 | 5.81 ± 0.30 | 0.19 ± 0.06 | 15.73 ± 1.56 | 4.47 ± 0.07 |
0.63 | 67.18 ± 0.66 | 7.43 ± 0.15 | 4.80 ± 0.38 | N.D | 20.58 ± 1.13 | 3.26 ± 0.05 | |
1.25 | 69.04 ± 1.04 | 7.56 ± 0.33 | 5.28 ± 0.44 | N.D | 19.12 ± 1.53 | 3.61 ± 0.08 | |
2.50 | 69.20 ± 1.48 | 7.44 ± 0.36 | 5.44 ± 0.34 | N.D | 17.92 ± 1.58 | 3.86 ± 0.09 | |
3.75 | 69.96 ± 0.43 | 7.78 ± 0.06 | 5.31 ± 0.18 | N.D | 15.95 ± 0.36 | 4.39 ± 0.02 | |
0 | 73.69 ± 2.35 | 5.52 ± 0.23 | 4.64 ± 0.20 | 2.00 ± 0.32 | 14.15 ± 2.26 | 5.21 ± 0.30 | |
Bio-char | 0.63 | 63.34 ± 2.33 | 4.99 ± 0.14 | 4.42 ± 0.12 | 2.88 ± 0.42 | 24.36 ± 2.18 | 2.60 ± 0.96 |
1.25 | 52.41 ± 2.87 | 4.34 ± 0.25 | 3.75 ± 0.26 | 4.17 ± 0.39 | 35.33 ± 3.30 | 1.48 ± 0.11 | |
2.50 | 45.60 ± 7.5 | 3.97 ± 0.57 | 3.30 ± 0.46 | 4.27 ± 0.86 | 42.86 ± 9.18 | 1.06 ± 0.27 | |
3.75 | 41.90 ± 5.00 | 3.73 ± 0.37 | 2.89 ± 0.32 | 3.76 ± 0.21 | 46.15 ± 5.75 | 0.91 ± 0.17 |
As for the bio-char, it is know that part of the catalyst remained on it, and that the generated bio-crude is free of catalyst, but further analyses are needed to determine the amount of catalyst that remains in the aqueous and solid phases. As detailed in Tables 1 & 3, the production of bio-char was slightly increased from 11.9 ± 0.0% (0 wt% of UNCFO) to 13.9 ± 0.0% (3.75% of UNCFO), whereas the amount of carbons fixed in the solid residues decreased from 73.6 ± 2.3% to 41.9 ± 5.0%, respectively. This signifies that the decrease of the carbon percentage in the bio-char is related to the increase in the carbon content in the bio-crude. The bio-char also exhibits a more aromatic nature and a low fixed carbon value by increasing the loading of UNCFO. Analogously, the slight decrease (∼5%) in the oxygen percentage of the bio-crude is associated to the reduction of polyketones and ketones. Similar findings were reported by Jena et al. working with a heterogeneous NiO catalyst via HTL process of S. platensis.26 Compared to the bio-crude produced using homogeneous catalysts, they found a reduction of 6% in the oxygen content. The removal of UNCFO from the bio-char to further exploit its superior catalytic properties and degree of reusability will be the subject of a subsequent work, as well as the performance of the UNCFO-free as-generated bio-char as a potential fertilizer.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr04404a |
This journal is © The Royal Society of Chemistry 2015 |