Issue 45, 2015
From the journal:

Chemical Communications

The development of a nucleus staining fluorescent probe for dynamic mitosis imaging in live cells†

Krishna Kanta Ghosh,‡a   Yun-Mi Jeong,‡b   Nam-Young Kang,b   JungYeol Lee,a   Wan Si Yan Diana,b   Jun-Young Kim,b   Jaeduk Yoo,a   Dohee Kim,cd   Yun Kyung Kim*ce  and  Young-Tae Chang*ab  

* Corresponding authors

a Department of Chemistry & MedChem Program of Life Sciences Institute, National University of Singapore, 117543 Singapore
E-mail: chmcyt@nus.edu.sg
Fax: +65-6779-1691
Tel: +65-6516-7794

b Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios Building, 138667 Singapore

c Center for Neuro-medicine, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea

d Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea

e Department of Biological Chemistry, University of Science and Technology, Daejon, Republic of Korea

Abstract

A low-toxicity nucleus staining fluorescent probe, CDb12, was developed for real time mitosis imaging in live cells. CDb12 was identified by unbiased high-throughput imaging-based screening of a new xanthone library (AX). Unlike the conventional Hoechst dye, the low toxicity of CDb12 allows long term monitoring of cell division over more than one cell cycle.

Graphical abstract: The development of a nucleus staining fluorescent probe for dynamic mitosis imaging in live cells

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Article information

DOI
https://doi.org/10.1039/C5CC02295A
Article type
Communication
Submitted
19 Mar 2015
Accepted
20 Apr 2015
First published
24 Apr 2015

Chem. Commun., 2015,51, 9336-9338

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The development of a nucleus staining fluorescent probe for dynamic mitosis imaging in live cells

K. K. Ghosh, Y. Jeong, N. Kang, J. Lee, W. Si Yan Diana, J. Kim, J. Yoo, D. Kim, Y. K. Kim and Y. Chang, Chem. Commun., 2015, 51, 9336 DOI: 10.1039/C5CC02295A

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