Issue 20, 2015

Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections

Abstract

Ion mobility spectrometry techniques (IMS and IMS-IMS) combined with collision-induced dissociation (CID) and mass spectrometry (MS) are used to investigate the structures of singly-lithiated carbohydrate isomers. With the exception of some favorable cases, IMS-MS analyses of underivatized carbohydrates reveal that most isobaric precursor ions have similar collision cross sections (ccs). In contrast, ccs values for isomeric fragment ions obtained by IMS-CID-IMS-MS analysis are often different, and thus appear to be useful as a means of distinguishing the isomeric precursors. We report values of ccs (in He) for precursor- and associated-fragment ions for three monosaccharide isomers (glucose, galactose and fructose), ten disaccharide isomers (sucrose, leucrose, palatinose, trehalose, cellobiose, β-gentiobiose, isomaltose, maltose, lactose and melibiose), and three trisaccharide isomers (raffinose, melezitose and maltotriose). These values are discussed as a means of differentiating precursor carbohydrates.

Graphical abstract: Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections

Article information

Article type
Paper
Submitted
28 Apr 2015
Accepted
07 Aug 2015
First published
07 Aug 2015

Analyst, 2015,140, 6922-6932

Author version available

Investigating carbohydrate isomers by IMS-CID-IMS-MS: precursor and fragment ion cross-sections

M. M. Gaye, R. Kurulugama and D. E. Clemmer, Analyst, 2015, 140, 6922 DOI: 10.1039/C5AN00840A

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