Dan Zhu,
Zhuyuan Wang*,
Shenfei Zong,
Hui Chen,
Peng Chen and
Yiping Cui*
Advanced Photonics Center, Southeast University, 2# Sipailou, Nanjing 210096, Jiangsu, China. E-mail: cyp@seu.edu.cn; wangzy@seu.edu.cn; Fax: +86-25-83790201; Tel: +86-25-83792470
First published on 24th October 2014
A new optical encoding approach, the wavenumber–intensity joint surface enhanced Raman scattering (SERS) spectral encoding method, was demonstrated by using silver nanoparticles with a core–shell structure. Using three kinds of Raman reporters, 1,4-benzenedithiol (BDT), 2-naphthalenethiol (2-NAT) and 4-methoxythiophenol (4-MT), which were self-assembled on the surfaces of a silver core, 19 codes with distinguished spectral characteristics have been achieved. By conjugating specific antibodies to silver nanoparticles with a certain code, the potential application of such an encoding system in tumor cell targeting has been investigated. The high selectivity of the assay indicates that the joint encoding method could be developed as a powerful tool for high-throughput bioanalysis in the future.
In a SERS-based encoder, labels (Raman reporters) are usually adsorbed onto the roughened metal surfaces to generate strong SERS signals.15–18 By combining different kinds of Raman reporters, nanoprobes with many optical codes can be achieved.19–22 Thus, parallel detection of multiplex target molecules could be performed with several kinds of Raman reporters. Moreover, the characteristic spectral signatures from various distinct Raman labels require only a single laser excitation wavelength. However, with the increasing number of required codes, the Raman reporters involved in the encoded nanoprobes may become rather complicated. Although the amounts of available Raman reporters are abundant, similar structures of these reporter molecules make it difficult to be distinguished quite well, because many reporters have the similar chemical bonds. The above factors restrict the number of codes that can be realized practically. As far as we know, less than 10 kinds of SERS codes have been obtained.10,23 Therefore, an optical encoding method with increased encoding capacity is still required.
Here, a wavenumber–intensity joint SERS spectral encoding method has been demonstrated, where numerous different SERS characteristic signatures can be obtained using only a few Raman reporters. Silver nanoparticles (Ag NPs) were chosen as the SERS substrates while 1,4-benzenedithiol (BDT), 2-naphthalenethiol (2-NAT) and 4-methoxythiophenol (4-MT) were selected as Raman reporters. By tuning the molar ratios of three kinds of Raman reporters, SERS spectra with differences in wavenumber or intensity ratio were acquired. In total, 19 different ternary combinations were achieved. The multiplexing capacity is determined not only by the type of the Raman labels but also by the stoichiometric ratio as an additional parameter. Subsequently, these Raman reporter functionalized-silver nanoparticles were coated with a dense layer of silica for further surface modification. Furthermore, after being conjugated with specific antibodies, the SERS encoded nanoprobes could be utilized for tumor cell targeting.
In the targeting experiments, SKBR3 (HeLa) and MRC5 cells were seeded into tissue culture dishes (Corning) and incubated for 24 h. Then the targeting nanoprobe solution and the nontargeting nanoprobe solution were added to the cell culture dish. An hour later, the culture media were discarded and the culture dishes were gently washed with PBS buffer three times before SERS measurements. For each condition, one spectrum was collected in each cell and the measured SERS spectra of 10 cells were used to obtain an average SERS spectrum.
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Scheme 1 (A) Schematic preparation of the encoded SERS nanoprobes; (B) color-coded SERS spectra of individual Raman reporter: BDT (red), 2-NAT (green) and 4-MT (blue). |
In our experiments, the silver nanospheres with a diameter of around 60 nm were prepared and shown in Fig. 1A, consistent with the DLS data shown in Fig. S1 (ESI†). Subsequently, the Raman reporters were adsorbed onto the surfaces of the Ag NPs through their thiol groups. Here, we chose three kinds of commercially available aromatic compounds, i.e. 1,4-benzenedithiol (BDT), 2-naphthalenethiol (2-NAT) and 4-methoxythiophenol (4-MT) as the Raman reporters. These Raman reporters all have large Raman scattering cross-sections and exhibit intense SERS signals (Scheme 1B). As reported previously, these Raman reporters can self-assemble on the Ag nanosphere surfaces to form monolayers.27,28
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Fig. 1 (A) TEM image of Ag NPs; (B) TEM image of Ag@reporter@SiO2 NPs; (C) extinction spectra of Ag NPs and Ag@reporter@SiO2 NPs. |
After the Ag@reporter NPs were prepared, an outer silica layer was obtained by a modified Stöber method.25 In order to get a more uniform silica shell, the Ag@reporter NPs were first coated by the amphiphilic, nonionic polymer PVP and then transferred to ethanol solution. Further, after the injection of a proper amount of ammonia and TEOS, a smooth silica shell with a thickness of ∼35 nm was formed (Fig. 1B). Fig. 1C shows the extinction spectra of both Ag NPs and Ag@reporter@SiO2 NPs. Comparing the two curves in Fig. 1C, it can be observed that the silica coating process induced a red shift of the extinction peak about 50 nm of the Ag NPs due to the increase in the refractive index.29
This result corresponds well with the fact that only a single Ag NP was encapsulated in each silica nanoparticle (Fig. 1B), because no obvious extinction band is observed in the long wavelength range, which was usually related with the aggregation of Ag NPs.30
SERS spectra of three different nanoprobes as Ag@BDT, Ag@2-NAT and Ag@4-MT are shown in Fig. 2 with the chemical structures of three molecules. As shown in Fig. 2, all of the three kinds of SERS nanoprobes display strong and unique spectroscopic signatures. Typically, BDT shows two dominant SERS peaks at 1565 and 1180 cm−1, which are assigned to modes ν8a and ν9a. The Raman band at 1080 cm−1 is ascribed to the ν1 fundamental in Fermi resonance with a combination mode consisting of ν6a + ν7a and that at 730 cm−1 is attributed to modes ν7a.31,32 Moreover, 2-NAT exhibits characteristic SERS peaks at 1621 and 1378 cm−1 dominated by ring modes. Besides, the Raman band at 1080 cm−1 is attributed to the C–H bending and that at 767 cm−1 is ascribed to the ring deformation.33,34 Meanwhile, 4-MT presents two at 1081 and 1591 cm−1, which are assigned to C–C stretching vibration. The Raman band at 794 cm−1 is ascribed to the C–H bending.31–35 Importantly, when the mixture of BDT, 2-NAT and 4-MT was used, signal peaks at 730, 767 and 794 cm−1 can be observed simultaneously without spectroscopic overlap. Thus, it should allow the easy identification of the corresponding targets in a mixture with multiplex analytes. In the following experiments, the above three SERS bands are used for encoding.
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Fig. 2 SERS spectra of three SERS nanoprobes and the chemical structures of encoded Raman reporters: (a) BDT, (b) 2-NAT and (c) 4-MT. |
Using the existence information of SERS peaks at 730, 767 and 794 cm−1, in a traditional wavenumber encoding system, only 7 codes can be generated employing those three Raman reporters. However, on the basis of the combined information of wavenumber and intensity, a total of 19 codes were achieved experimentally with distinguished optical spectral signatures, whose components, structures, and measured optical spectra are listed in Fig. 3. To facilitate encoding, the intensity of characteristic peaks is divided into three levels labeled by number 0, 1 and 2, respectively. The number 0 represents no signal at a certain wavenumber, while the number 1 or 2 mean signal intensity information at a particular wavenumber. For instance, the characteristic peak at 730 cm−1 from BDT possesses approximately equal intensity of the 2-NAT peak at 767 cm−1 while no peak at 794 cm−1 corresponding to 4-MT appears, which results in a spectrum with code ‘110’. In the case of code ‘120’, the intensity of 730 cm−1 is only half of that at 767 cm−1. Another case is that the intensity of 730 cm−1 is twice of that at 767 cm−1. As a result, a spectrum with code ‘210’ is obtained.
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Fig. 3 Codes, structures and measured spectra of the synthesized 19 SERS encoders. The spectra are only shown from wavenumber 600 to 900 for clarity. |
According to the encoding rule mentioned above, SERS encoders can be classified as nanoprobes containing one-, two-, or three-components. In the first case, Ag NPs were functionalized with each reporter individually. Moreover, the obtained codes, named as 100, 010 and 001, corresponded directly with each fingerprint signature. Under second situation, the SERS encoders with BDT/2-NAT (Code ‘110’, ‘120’ and ‘210’), BDT/4-MT (Code ‘101’, ‘102’ and ‘201’) and 2-NAT/4-MT (Code ‘011’, ‘012’ and ‘021’) provided nine distinguished SERS spectra. Finally, SERS encoders functionalized with three types of Raman reporters together were acquired. Spectra of codes (‘111’, ‘112’, ‘121’, ‘211’, ‘122’, ‘212’, ‘221’) exhibit the SERS bands of BDT at 730 cm−1, 2-NAT at 767 cm−1 and 4-MT at 794 cm−1 simultaneously, but display differences in relative intensities of SERS signals. As a result, SERS encoders containing different amount of BDT, 2-NAT and 4-MT can be spectrally distinguished from each other.
Although, in principle, the same number of codes can be acquired by using more kinds of Raman reporters in a wavenumber-dependent SERS encoding system, the preparation process will become rather complicated or even unpractical because of the problems of spectral overlap or limited available types of Raman reporters. However, in the wavenumber–intensity joint SERS encoding approach, enlarging the number of codes is much easier and more feasible due to the following reason. One thing worth noting is that as an example, the above SERS encoders are acquired only with three different levels of signal intensity. Actually, a much larger number of codes will be generated when more levels of intensity are introduced, which can be realized by controlling the amount of each reporters more accurately.
Since overexpression of epidermal growth factor receptor 2 (HER2) has been found in SKBR3 cells,36–38 the anti-HER2 antibody decorated NPs can be internalized prodigiously by the SKBR3 cells through receptor-mediated endocytosis.39 Thus, SKBR3 cells which overexpress HER2 receptors on their membranes were chosen as the model target cancer cells, while anti-HER2 antibody free probes and HER2 receptor negative human embryonic lung fibroblasts (MRC5) cells were chosen as the negative controls to examine the targeting ability of the nanoprobe with code ‘100’. After being incubated with the corresponding nanoprobes, SERS spectra from nanoprobes in three different conditions were all collected and shown in Fig. 4. SKBR3 cells incubated with the targeting probes (Fig. 4C and D) generated much stronger SERS signals than SKBR3 cells incubated with the anti-HER2 antibody free control probes (Fig. 4E and F) and MRC5 cells incubated with the targeting probes (Fig. 4G and H). According to Fig. 4B, SERS signals measured from SKBR3 cells incubated with the targeting nanoprobes are about 2.4 times higher than those from the two controls. The signal to noise ratio (SNR) at 730 cm−1 under the three different conditions were 1.089, 1.039 and 1.038, respectively, supporting that more targeting nanoprobes were taken up efficiently by the HER2 receptor over-expressed SKBR3 cells. These results showed that the proposed targeting nanoprobe can recognize HER2 overexpressed cancer cells with a high selectivity and improve the cellular uptake of the targeting nanoprobes through the HER2-receptor mediated endocytosis.
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Fig. 4 (A) SERS spectra of the nanoprobes in living cells under different conditions; for each situation, the SERS spectra were collected from 10 randomly selected cells and average results were presented; (B) SERS intensities of the bands at 730 cm−1 correspond to the Fig. 4A, the error bars represent the standard deviation of 10 measurements; (C and D) SERS mapping of SKBR3 cells incubated with the targeting probe; (E and F) SERS mapping of SKBR3 cells incubated with the anti-HER2 antibody free probe; (G and H) SERS mapping of MRC5 cells incubated with the targeting probe. |
Once the anti-HER2 antibody-conjugated SERS encoder with code ‘100’ was determined to be capable of targeting SKBR3 cells, the general applicability of the tumor cell targeting was investigated by using transferrin-conjugated SERS encoder with code ‘010’ to target HeLa cells (Scheme 2B). In the experiments, HeLa cells were chosen as the model target cancer cells due to its abundant transferrin receptors. Transferrin free probes and transferrin receptor negative MRC5 cells were chosen as the negative controls. The experimental results showed that the SERS encoder with code ‘010’ exhibited fine targeting performance as well as the SERS encoder with code ‘100’ (Fig. S2, ESI†). Therefore, the presented SERS encoder can indeed target multiple cancer cell types.
By selecting the SERS encoder with different optical codes, which are functionalized with different targeting antibodies, more SERS encoders with targeting ability for multiplex tumor cells can be achieved. Moreover, the SERS encoded nanoprobe is not limited to tumor cell targeting since its encoding method can be utilized in immunoassay as well as DNA detection. It should be noted that utilizing our demonstrated SERS encoding method, 19 different analytes could be simultaneously detected using only three kinds of Raman reporters. The wavenumber and intensity joint SERS encoding would have a great potential in encoding techniques and own promising applications in multiplex detection.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra11522h |
This journal is © The Royal Society of Chemistry 2014 |