Akihiro
Okamoto
a,
Koichiro
Saito
a,
Kengo
Inoue
b,
Kenneth H.
Nealson
c,
Kazuhito
Hashimoto
*a and
Ryuhei
Nakamura
*d
aDepartment of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. E-mail: hashimoto@light.t.u-tokyo.ac.jp
bInterdisciplinary Research Organization, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
cDepartments of Earth Sciences and Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA
dBiofunctional Catalysts Research Team, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. E-mail: ryuhei.nakamura @riken.ac.jp
First published on 12th February 2014
Geobacter species are among the most efficient current-producing bacterial species, yet their electron-transfer mechanisms have been scarcely investigated at the molecular level. Here, we provide evidence that Geobacter cells secrete and utilize riboflavin as a bound-cofactor in outer-membrane c-type cytochromes. This finding highlights the potential roles of riboflavin as a major electron carrier in current production.
Broader contextGeobacter sulfurreducens is an iron-reducing bacterium that has a significant content of c-type cytochromes (c-Cyts) in an outer-cell membrane (OM) and bacterial filaments, drawing keen attention as a model microorganism for the research of microbial fuel cells and bioremediation technology. Concerned with the ability of Geobacter to transport electrons to electrodes in microbial fuel cells, processes termed extracellular electron transport (EET), a great deal of research has been focused on identifying the molecular mechanisms behind the bacterial current generation. Herein, we report the first evidence that Geobacter utilizes self-secreted flavins as a redox cofactor in OM c-Cyts. Using a highly sensitive voltammetry technique, the key redox signal for the Geobacter EET was identified in vivo. Experiments using a mutant unable to produce OM c-Cyts, together with the spectroscopic and LC-MS analyses, revealed that the noble redox signal is assigned to the bound riboflavin associated with OM c-Cyts containing reduced hemes. As the heme redox state reflects a balance between the electron input from respiration and the output by EET, the present study not only significantly contributes to the molecular understanding of EET, but also highlights the microbial capability of utilizing self-secreted riboflavin as a regulator for intracellular redox homeostasis. |
Over the last decade, several mechanisms for microbial EET have been proposed, including indirect electron transfer via redox-active organic electron shuttles9,10 and direct electron transfer by c-type cytochromes (c-Cyts) located in the outer membrane (OM)11 or on nanometer-scale bacterial filaments.12,13 In indirect EET, microbial cells are able to perform electron transfer without the necessity of direct contact with solid-phase electron acceptors. Thereby, as the surface area of the solids is limited, EET via self-secreted, naturally occurring or artificially supplemented soluble electron shuttles appears to be a major pathway for electron transfer to solids.10 However, such soluble redox molecules are apparently not involved in the EET process of Geobacter sulfurreducens,14 which is the most efficient current-producing microorganism characterized to date. This conclusion was reached after it was shown that the exchange of supernatants to fresh medium in an electrochemical reactor did not impair current production by G. sulfurreducens as it did in other microorganisms.14 Recently, we reported that cell-secreted redox molecules, such as flavins, have a high affinity for OM c-Cyts with reduced hemes in Shewanella oneidensis MR-1.15 This flavin binding to OM c-Cyt scaffolds was found to facilitate a one-electron redox reaction via semiquinone, resulting in a 103- to 105-fold enhancement of the EET rate compared to free flavin.15 Based on this finding, we speculated that Geobacter cells might also use self-secreted redox molecules as redox cofactors to promote EET. If true, the excreted molecules should not act as a diffusion-based electron shuttle in G. sulfurreducens, and thereby the supernatant exchange could not impair current production by G. sulfurreducens.14 This possibility is supported by the fact that, similar to S. oneidensis MR-1,16G. sulfurreducens have genes for both flavin biosynthesis and secretion (Fig. S1†), although their functions have not been confirmed.
In this paper, we report that G. sulfurreducens secrete flavin that contributes to EET at the cell/electrode interface, highlighting the crucial role of flavin redox cycling for efficient EET in this species.
To directly examine the secretion of flavin by G. sulfurreducens, a supernatant solution of anaerobically grown cultures was subjected to spectroscopic measurements (Fig. 1). Geobacter cells were grown in a defined medium supplemented with acetate (20 mM) as an electron donor and fumarate (80 mM) as an electron acceptor. As shown in Fig. 1a and b, both the peak intensities of emission at 525 nm and excitation at 370 and 440 nm increased when the growth curve of cells was in a sigmoidal phase. The emission and excitation spectral peaks were identical to those observed in the spectra of riboflavin (RF) and flavin mononucleotide (FMN) solutions without cells (Fig. S2a and b†), suggesting that G. sulfurreducens cells secrete flavin species during growth. In support of these data, mass chromatography patterns of the cell culture supernatant measured in selective ion monitoring (SIM) mode at m/z 375.35 identified that RF was present at a concentration of 100 nM, although FMN at m/z 455.34 was under the detection limit (Fig. 1c and S3†). These findings provide evidence that G. sulfurreducens secrete RF at concentrations comparable to that observed in cultures of anaerobically grown S. oneidensis MR-1.17
The contribution of secreted RF for EET was examined by adding a concentrated RF solution during the electrochemical cultivation of G. sulfurreducens. Current production (Ic) was measured using a cell suspension with an optical density at λ = 600 nm (OD600) of 0.2 cultured with 10 mM acetate as the sole electron source on an indium-tin oxide (ITO) electrode at a poised potential of +0.4 V (vs. SHE) (ESI†). At several time points during the course of microbial current production, differential pulse (DP) voltammetry was conducted (Fig. 2a). Three peaks were observed in DP voltammograms after 46 h (Fig. 2b), but only the redox peak current at −0.2 V (I−0.2) increased with time, whereas the peak current of the other redox peaks decreased inversely with time (Fig. 2b). When 1.0 μM RF was added to Geobacter cells on the electrode during current production under the same conditions as above, the Ic rapidly increased by ∼10% (Fig. S4†). Furthermore, as the addition of RF also caused an increase in I−0.2 (Fig. S5†), it is possible to assign the redox peak at −0.2 V to the redox cycling of RF. This result also indicates that Geobacter cells utilize self-secreted RF for EET, even before the addition of exogenous RF.
We plotted I−0.2 against Ic to quantify the contribution of the redox species at −0.2 V to EET. As shown in Fig. 2, Ic exhibited a positive correlation with I−0.2, as a fitted line passed through the point of origin with a high correlation coefficient (r2 = 0.998). Importantly, this trend was observed both before and after the addition of RF, and even after the addition of malonic acid as a metabolic inhibitor (Fig. 2d and S4†). These results confirm the assignment of the peak potential (Ep) at −0.2 V to the redox cycling of RF, and demonstrate that respiratory current generation by G. sulfurreducens is strongly coupled with the amount of electrochemically active RF. In addition, FMN exhibited a similar effect on the Ic and peak current to that seen with RF, but at a different Ep (Fig. S6†). Upon the addition of FMN, both the peak current at −0.175 V (I−0.175) and Ic increased, and a fitted line for the plot of I−0.175 and Ic passed through the point of origin (Fig. S6†), demonstrating the capability of G. sulfurreducens to utilize FMN as an efficient electron carrier. Together, these data provide evidence for the involvement of RF and/or FMN as important electron carriers at the interface between Geobacter cells and ITO electrodes.
The measured Ep in the DP voltammogram of RF and FMN solutions in the absence of cells significantly differ from those observed in Geobacter biofilms (Fig. S7†), suggesting that both flavins alter their redox properties, as is reported for flavins bound to OM c-Cyts in S. oneidensis MR-1.15 Assuming one of the c-Cyts located on bacterial filaments or embedded in the OM interacts with flavins as bound cofactors in G. sulfurreducens, insight into the location of c-Cyts that bind RF is important to determine the predominant EET pathway in this species. Fig. 3 shows scanning electron microscopy (SEM) images of G. sulfurreducens cells attached to the ITO electrode after 50 h of electrode cultivation. Neither filament-like assemblages nor multilayer biofilms were formed on the electrode surface, corresponding to a previous report where thick biofilms of G. sulfurreducens cells with Cyt-bound filaments required more than 4 days of electrochemical cultivation.18 Therefore, it appears that the EET process mediated by the redox cycling of flavins observed in Fig. 2 is the consequence of the activation of c-Cyts located primarily on the OM surface, as opposed to those located on the surface of conductive bacterial filaments.
To examine the specific interactions between flavins and OM c-Cyts, we used a mutant strain (ΔomcBEST) of G. sulfurreducens lacking OmcB, OmcE, OmcS, and OmcT, which are the major multi-heme c-Cyts localized on the OM of Geobacter.19 When cultured at +0.4 V in the presence of 10 mM acetate, current production by the ΔomcBEST strain was highly impaired (Fig. S8†). In addition, the peak position and Ip of RF displayed a negative shift of 75 mV and 20-fold decrease, respectively, compared to those of the wild-type (WT) strain (Fig. 4a and S9†). Under FMN-supplemented conditions, the Ep of ΔomcBEST also exhibited a 50 mV negative shift in the DP voltammogram and a large Ip decrease compared to that of WT (Fig. S10†). These significant effects on Ep observed for the ΔomcBEST strain demonstrate that both FMN and RF associate with OmcB, OmcE, OmcS, or OmcT c-Cyt proteins to enhance the rate of EET in WT G. sulfurreducens cells. Such specific association of RF with OM c-Cyts of G. sulfurreducens cells was also confirmed by the fluorescence analysis of the OM fraction,20 where less flavin contents were observed for the OM fraction of ΔomcBEST cells compared with that of WT (Fig. S11†). However, in the system without omcBEST genes, the RF peak current at −0.125 V and Ic still showed a positive correlation with a comparable slope value to WT (Fig. S9c†). This result suggests that, in addition to omcBEST proteins, other types of OM c-Cyts in the Geobacter WT strain associate with RF to mediate EET (Fig. 4). This situation differs from the EET process reported for S. oneidensis MR-1, which is unable to bind FMN in the absence of a single binding protein (MtrC).14 This finding is also consistent with the wider variety of c-Cyts encoded in the genome of G. sulfurreducens compared to that of S. oneidensis MR-1.21 Together, these results confirm that RF and FMN associate with OM c-Cyts of G. sulfurreducens and serve as redox cofactors (Fig. 4), and that Geobacter is more flexible with respect to flavin uptake and binding mechanisms than S. oneidensis MR-1. In addition, because the peak current for bound-RF decreased when Ic was reduced by the addition of a metabolic inhibitor (Fig. 2d), reduced hemes in OM c-Cyts of G. sulfurreducens play an important role in the interaction with flavins, as has also been reported in MR-1.15
Footnote |
† Electronic supplementary information (ESI) available: Figures and methods. See DOI: 10.1039/c3ee43674h |
This journal is © The Royal Society of Chemistry 2014 |