Issue 2, 2015

Screening and analyzing the potential bioactive components from rhubarb, using a multivariate data processing approach and ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry

Abstract

The root of Rheum palmatum L., known as rhubarb, has been widely used in the treatment of obstipation, gastrointestinal indigestion and other diseases in China and other Asian countries for thousands of years. However, the constituents absorbed into the blood after oral administration of rhubarb remain unknown. Here, a sensitive and rapid method using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS) technology combined with a multivariate data processing approach (MDPA) was established to investigate the absorbed constituents in rats after oral administration of rhubarb. Chromatographic fingerprints of the rhubarb samples were firstly established in the negative mode in vitro and in vivo, with 80 compounds found in rhubarb and 41 compounds found in rat plasma after oral administration of rhubarb. Of the 80 compounds detected in vitro, 78 were tentatively characterized. Of the 41 compounds identified in rat plasma, 19 were the original form of compounds absorbed from the 30 compounds detected in vitro, and 11 were the metabolites of compounds that existed in rhubarb. The integrative UPLC/Q-TOF-MS/MS and MDPA method were successfully applied for the rapid determination of multiple components from rhubarb. Based on the significance of these results, this method demonstrated that this method was a useful technique for rapid screening and identifying bioactive components from complex herbal medicines.

Graphical abstract: Screening and analyzing the potential bioactive components from rhubarb, using a multivariate data processing approach and ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry

Article information

Article type
Paper
Submitted
22 Oct 2014
Accepted
30 Oct 2014
First published
31 Oct 2014

Anal. Methods, 2015,7, 650-661

Author version available

Screening and analyzing the potential bioactive components from rhubarb, using a multivariate data processing approach and ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry

D. Yang, G. Sun, A. Zhang, S. Fu and J. Liu, Anal. Methods, 2015, 7, 650 DOI: 10.1039/C4AY02506G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements