Using T–Hg–T and C–Ag–T: a four-input dual-core molecular logic gate and its new application in cryptography

Dingyi Tong, Haifeng Duan, Hejing Zhuang, Jungang Cao, Zhonglin Wei and Yingjie Lin*
Department of Chemistry, Jilin University, 130012 Changchun, China. E-mail: linyj@jlu.edu.cn; Fax: +86-431-85168398; Tel: +86-431-85168398

Received 25th August 2013 , Accepted 5th November 2013

First published on 7th November 2013


Abstract

A simple four-input dual-core (thymine & cytosine) logic gate was successfully developed that utilized a succinic imide labelled pyrene probe as the signal responser. Moreover, this molecular logic gate could be made into fluorescent paper and applied in the field of cryptography.


Molecular logic gates are an emerging interdiscipline, utilizing integrated circuits in electronics on a molecular scale.1 With the rapid development of logic gates, complex molecular circuits with more inputs, outputs and gate symbols are now arousing more and more interest.2 Although a variety of them have been manufactured, logic gates with more than three-inputs are still rare. The reason is that multiple-inputs make the molecular circuits more complicated and difficult to synthesize. One effective strategy is introducing DNA into the logic system. Many specific structures such as C–Ag–C, T–Hg–T and G-quadruplexes have been used for the construction of complex logic gates.3 Among them, thymine and cytosine are two important elements in many DNA logic systems with metal ion inputs. Besides, many groups set out to develop the application of these complex logic gates. These molecular logic gates have been applied in biosensing and diagnostics, prodrug activation and drug delivery/release, intelligent materials and molecular computers.4 Meanwhile, many groups have stepped into the field of cryptography and developed many new nano or other chemical code systems.5 However, most of them rely on the use of DNA, enzymes and specialist equipment. Thus, these encryptions and decryptions seemed tedious and time-consuming. In this paper, we tried to integrate the functions of thymine and cytosine in one small organic molecule. Then we used this logic gate to develop a more user-friendly code system.

In recent years, simulating the interaction between thymine and Hg2+, “T–Hg–T”-like fluorescent probes based on imide structures have been developed and applied in the sensing of Hg2+. However, only perylene bisimide and naphthalimide were reported.6 Both could bind to Hg2+ in a “T–Hg–T”-like style only. Therefore, they were only used in Hg2+-sensors and not made into molecular logic gates.

In medicinal chemistry, a novel and efficient method was found to connect an aromatic group with a succinic imide. The products synthesized by this method have been applied in drug research.7 Meanwhile, as examples of important fluorescent probes, pyrene derivatives have been widely used as sensors of pH, DNA, metal ions and so on.8 Herein, we chose pyrene as the fluorescent unit and synthesized PyI through a one-pot step without column chromatography (Scheme 1). Such a simple synthetic route is one of its advantages. For comparison, compound PhI was also synthesized. Unlike perylene bisimide and naphthalimide, the structure of PyI is not planar. So the intermolecular π–π stacking of PyI is not as easy and its aggregation-caused quenching effect was not significant. Actually, the fluorescence of PyI in an EtOH–H2O mixture (1[thin space (1/6-em)]:[thin space (1/6-em)]4, v/v) was obvious (Fig. S1). In addition, it had excellent solid fluorescence (Fig. S2). Consequently in Fig. 1, it was found that its fluorescence could be quenched by Hg2+ in a PBS buffer of pH 6.0, or by Hg2+ or Ag+ in a PBS buffer of pH 8.0. Meanwhile, there were no changes when other metal ions (K+, Na+, Mg2+, Ca2+, Mn2+, Zn2+, Cd2+, Ni2+, Pb2+, Cu2+, Fe2+, Fe3+, Co2+, Co3+, Al3+, Cr3+) were added under both pH conditions. Then the same experiment was performed in a PBS buffer of pH 6.13–7.89. In Fig. S3, we observed a different quenching effect by Hg2+ and Ag+ under different pH conditions.


image file: c3ra44650f-s1.tif
Scheme 1 Synthesis of PyI and PhI.

image file: c3ra44650f-f1.tif
Fig. 1 Fluorescence changes of PyI (5.0 μM) upon the addition of 1 equiv. of various metal ions (λex = 375 nm) in EtOH–PBS buffer (5 mM) (1[thin space (1/6-em)]:[thin space (1/6-em)]4, v/v) (a) pH = 6.0; (b) pH = 8.0. (λex = 375 nm).

Fig. S4 and S5 show the fluorescence spectra of 5 μM PyI after adding different concentrations of Hg2+ or Ag+. In the spectra, there is a rapid decrease in intensity at 500 nm before the addition of 0.5 equiv. Hg2+ or Ag+. The intensity was generally stable at even higher concentrations of Hg2+ or Ag+. Thus, we suggest that Hg2+ or Ag+ and PyI form mainly 1[thin space (1/6-em)]:[thin space (1/6-em)]2 complexes. It was also proved by a Job's plot experiment (Fig. S6). However, complete quenching required more Hg2+ at pH 6.0. The possible reason is that the binding process between Hg2+ and PyI occurs as shown in Scheme 2. Clearly, H+ was an obstacle to this reaction and more Hg2+ was needed to form this “T–Hg–T”-like complex.


image file: c3ra44650f-s2.tif
Scheme 2 The binding process between Hg2+ and PyI.

These above experiments were conducted for our next molecular logic gate. We chose Na2S as the inhibitor for Hg2+ and Ag+. Taking into account the complete quenching and HS existing in an acidic environment, we decided to use 5 μM PyI, 5 μM Hg(NO3)2, 5 μM AgNO3 and 15 μM Na2S as the inputs of our logic gate. For the inputs of Ag+, Hg2+ or S2−, we defined their presence as 1 and absence as 0. For the input of H+, we defined pH 6.0 as 1 and pH 8.0 as 0. The fluorescence intensity at 500 nm (demarcation line: 200) was defined as the output (1 or 0). Fig. 2a displays the possible input and output combinations. Its logic circuit is given in Fig. 2b. The results show that our molecular logic gate can do the same as a DNA logic system can. Besides, it introduced H+ as the inhibitor for Ag+. With 16 kinds of input combinations and 3 different kinds of gate symbols (INHIBITOR, OR and IMPLICATION), our logic gate is brief but not simple. In addition, our logic gate (only needs 5 μM Hg2+ or Ag+) can be easily seen by the naked eye (Fig. 2c). We also studied the reversibility of this logic gate. Fig. S8 shows the repeated switching behavior with alternating addition of (a) Hg2+ and S2− at pH 6.0; (b) Hg2+ and S2− at pH 8.0; (c) Ag+ and S2− at pH 8.0; (d) dilute HNO3 and NaOH in the presence of Ag+.


image file: c3ra44650f-f2.tif
Fig. 2 (a) Fluorescence intensity of PyI (5 μM) at 500 nm in the presence of different inputs, (λex = 375 nm); (b) the logic circuit of the system (INH = INHIBIT; IMP = IMPLICATION); (c) photos of the logic gate PyI (5 μM) in solution under a UV lamp (365 nm); (d) the conversion between cytosine-like and thymine-like structures and the stability of the T–Hg–T-like and the C–Ag–T-like structures at pH 6–8.

Next, we also needed to study the mechanism of the logic gate PyI. Firstly, the changes in the absorption spectra reflected the aggregation of fluorescent molecules. In Fig. S9, adding Hg2+ at pH 6.0 and adding Ag+ or Hg2+ at pH 8.0 could cause the reduction in its UV-Vis absorption peaks. Then the apparent association constants Kapp for Hg2+ and Ag+ were calculated using linear regression of the curves in Fig. S10 by the Hill equation. Positive cooperative binding (n > 1) was observed, which indicated that their binding strength to Ag+ or Hg2+ became stronger as another PyI binded.9

According to previous literature, it was obvious in our research that a succinic imide could also form a “T–Hg–T”-like structure. This structure proved to be stable between pH 6.0–8.0. However, different from the previous imide probes, it was very unusual that the same quenching was observed after adding Ag+ at pH 6.8–8.0. Based on the electrospray ionization-MS (ESI-MS) analysis, we chose PhI to study the interaction between this kind of imide structure and Ag+. In Fig. S20, the molecular ion peak at m/z 479.0179 [2(PhI − H+) + Ag+] could be found and suggested the formation of 1[thin space (1/6-em)]:[thin space (1/6-em)]2 complexes of Ag+ with this kind of imide. In the 1H NMR spectra of the complex of PhI-Ag-PhI, the imido proton peak (11.4 ppm) disappeared completely (Fig. S21). We supposed that this kind of imide structure might change into another structure to capture Ag+. Akira Ono's group reported that 5-fluorouracil could shift from a thymine structure to a cytosine structure and form the stable “C–Ag–T” structure under basic conditions.10 Due to the molecular conjugative effect, PyI could also become a cytosine-like structure (Fig. 2d). This structure was preferably present in a basic environment (Fig. S11 and Scheme S1). In an acidic environment, it was so difficult to combine Ag+ with thymine-like PyI that the fluorescence quenching by Ag+ could not be observed.

In our previous work, we have studied the addition of nitro compounds to open unsaturated double bonds.11 In this paper, there was also an unsaturated double bond in PyI. Ag+ or Hg2+ could tie two PyIs together and the fluorescence quenching was mainly caused by heavy atom enhanced inter-system crossing/spin–orbit coupling.12 What's more, this quenching effect might be transmitted to the pyrene unit through the conjugated double bond. To prove our hypothesis, we added 10 μL CH3NO2 into the system. Because the double bond was opened via a Michael reaction, this quenching effect was partially hindered. This caused enhanced fluorescence (Fig. 3a and b). However, due to the molecular conjugative effect, the cytosine-like structure was actually not a good substrate for a Michael reaction (Fig. 3c). In Fig. 3b, as expected, the change in the fluorescence intensity after adding CH3NO2 in the presence of Ag+ was about half of that in the presence of Hg2+. Different fluorescence lifetimes (t(PyI + CH3NO2) ≈ t(PyI + Hg2+ + CH3NO2) > t(PyI + Ag+ + CH3NO2)) also reflected the difference between these two reactions (Fig. S12). With the help of the Michael reaction, we verified the quenching mechanism.


image file: c3ra44650f-f3.tif
Fig. 3 Fluorescence changes of PyI (5.0 μM) upon the addition of 1 equiv. Hg2+ or Ag+ (λex = 375 nm) with or without 10 μL CH3NO2[thin space (1/6-em)]:[thin space (1/6-em)]EtOH–PBS buffer (5 mM) (1[thin space (1/6-em)]:[thin space (1/6-em)]4, v/v) (a) pH = 6.0; (b) pH = 8.0; (c) the reaction scheme between PyI–Hg–PyI or PyI–Ag–PyI with CH3NO2.

In addition, different from other common fluorescence logic gates, its solid fluorescence is so good that it could be made into fluorescent paper. Thus, we could use the fluorescent paper as the molecular logic gate. Meanwhile, we found that 16 kinds of inputs could lead to two outputs by PyI. The input could be represented by hexadecimal (0 ∼ F). We defined the function of the logic gate PyI as y = fPyI(x) (Fig. S13) and had a molecular code book as shown in Table 1. Therefore, it could be considered to be applied in the field of cryptography. Different from previous code systems, it looks more like asymmetric encryption. We could encrypt a message using the code book and decrypt it using chemical input tubes (Fig. S14 and Table S4). For example, a spy wrote a secret letter (ABCD 2346 EAF) on PyI fluorescent paper using the code book (Fig. 4). From it, the receiver could decrypt the message (0111 0100 001) using chemical input tubes (detailed steps in Table S3). Then decrypted using the Morse alphabet (Fig. S16), the letter really meant “JLU”. Thus, all the spy needed was the special paper to write on using the code book, without knowledge of the fluorescent dye in the paper. All the receiver needed to do was to decrypt using chemical input tubes without being clear about the code book and which chemicals were in the input tubes. By changing the chemical input tubes (Fig. S15), we could get 384 kinds of code systems. Compared with traditional encryption methods, this new molecular method is also very secure and reliable. Like never before, our logic gate PyI doesn’t require cryptographic computations, complicated operations, biochemical knowledge or specialist equipment. More importantly, the logic gate paper is easy to prepare and visible to the naked eye. This is the first report of its kind and could be a novel development direction for the application of molecular logic gates.

Table 1 Molecular code book of the logic gate PyI
Hexadecimal inputa Binary output
a Transcoded from the inputs of Fig. 2a.
2, 4, 6, A, E 0
0, 1, 3, 5, 7, 8, 9, B, C, D, F 1



image file: c3ra44650f-f4.tif
Fig. 4 (a) Encrypted using the code book (Table 1); (b) decrypted using chemical input tubes; (c) the details of the encryption and decryption.

In summary, with the idea of using T–Hg–T and C–Ag–T, we successfully synthesized a novel molecular logic gate based on succinic imide for H+, Ag+, Hg2+ and S2−. Owing to its molecular conjugative effect, the functions of cytosine and thymine were integrated into one system. Different from common molecular logic gates with several binding sites, one of this dual-core (thymine and cytosine) logic system's advantages is its simple structure and synthesis. With these two cores present, this new dual-core logic gate can handle both Ag+ and Hg2+ without interference from other metal ions. In addition, the logic gate is visible to the naked eye and can be made into useful and portable fluorescent paper. Then taking advantage of its hexadecimal inputs and binary outputs, we used the molecular logic gate PyI in the field of cryptography. We believe that the sensitive, recycled, visualized and simple logic gate has broad prospects in cryptographic applications.

Acknowledgements

We thank the Facility Center for the College of Chemistry in Jilin University for assistance with the optical measurements.

Notes and references

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Footnote

Electronic supplementary information (ESI) available: Experimental details, NMR and HRMS spectra, optical spectra and cryptography details. See DOI: 10.1039/c3ra44650f

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