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The effect of pH on the spectral and redox properties of model GFP synthetic chromophores (Cros) in a universal buffer system was investigated. The Cro hydroxy-derivatives demonstrated a Nernst-type electrochemical dependence of the anodic potential within the pH 2–8 range. Analogous studies on various fluorescent proteins were unsuccessful.

Graphical abstract: Spectral and redox properties of the GFP synthetic chromophores as a function of pH in buffered media

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