Issue 4, 2013

Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system

Abstract

Recently new lysine modifications were detected in histones and other proteins. Using the pyrrolysine amber suppression system we genetically inserted three of the new amino acids ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine site specifically into histone H3. The lysine at position 9 (H3 K9), which is known to be highly modified in chromatin, was replaced by these unnatural amino acids.

Graphical abstract: Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system

Supplementary files

Article information

Article type
Communication
Submitted
29 Oct 2012
Accepted
14 Nov 2012
First published
15 Nov 2012

Chem. Commun., 2013,49, 379-381

Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system

M. J. Gattner, M. Vrabel and T. Carell, Chem. Commun., 2013, 49, 379 DOI: 10.1039/C2CC37836A

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