Issue 10, 2012
From the journal:

Molecular BioSystems

Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range†‡

Sigrid Milles,a   Christine Koehler,a   Yann Gambin,b   Ashok A. Deniz*c  and  Edward A. Lemke*a  

* Corresponding authors

a EMBL, Structural and Computational Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany
E-mail: lemke@embl.de
Fax: +49 6221 387 536
Tel: +49 6221 387 537

b The University of Queensland, Institute for Molecular Bioscience, St Lucia, Brisbane, Australia
Fax: +61 7 334 62101
Tel: +61 7 334 62334

c The Scripps Research Institute, Department of Molecular Biology, 10550 N Torrey Pines Rd, La Jolla, USA
E-mail: deniz@scripps.edu
Fax: +1 858 784 9067
Tel: +1 858 785 9868

Abstract

Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.

Graphical abstract: Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2MB25135C
Article type
Communication
Submitted
05 Apr 2012
Accepted
08 Jun 2012
First published
13 Jun 2012

Mol. BioSyst., 2012,8, 2531-2534

Permissions

Request permissions

Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

S. Milles, C. Koehler, Y. Gambin, A. A. Deniz and E. A. Lemke, Mol. BioSyst., 2012, 8, 2531 DOI: 10.1039/C2MB25135C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements