Erica Ueda, Florian L. Geyer, Victoria Nedashkivska and Pavel A. Levkin
Lab Chip, 2012,12, 5218-5224
DOI:
10.1039/C2LC40921F,
Paper
We describe a one-step method for creating thousands of isolated pico- to microliter-sized droplets with defined geometry and volume. Arrays of droplets are instantly formed as liquid moves along a superhydrophilic–superhydrophobic patterned surface. Bioactive molecules, nonadherent cells, or microorganisms can be trapped in the fully isolated microdroplets for high-throughput screening, or in hydrogel micropads for screening in 3D microenvironments.