Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore

Lorenzo Franceschini; Ellina Mikhailova; Hagan Bayley; Giovanni Maglia

doi:10.1039/C1CC16124E

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Nucleobase recognition at alkaline pH and apparent pK_a of single DNA bases immobilised within a biological nanopore†‡

Lorenzo Franceschini,^a Ellina Mikhailova,^b Hagan Bayley^b and Giovanni Maglia*^a

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* Corresponding authors

^a Department of Chemistry, University of Leuven, 3001, Heverlee, Leuven, Belgium
E-mail: giovanni.maglia@chem.kuleuven.be
Tel: +32 16 327 696

^b Department of Chemistry, University of Oxford, Oxford, UK

Abstract

The four DNA bases are recognized in immobilized DNA strands at high alkaline pH by nanopore current recordings. Ionic currents through the biological nanopores are also employed to measure the apparent pK_a values of single nucleobases within the immobilised DNA strands.

This article is part of the themed collection: Emerging Investigators 2012

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Article information

DOI: https://doi.org/10.1039/C1CC16124E
Article type: Communication
Submitted: 01 Oct 2011
Accepted: 07 Nov 2011
First published: 17 Nov 2011

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Chem. Commun., 2012,48, 1520-1522

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Nucleobase recognition at alkaline pH and apparent pK_a of single DNA bases immobilised within a biological nanopore

L. Franceschini, E. Mikhailova, H. Bayley and G. Maglia, Chem. Commun., 2012, 48, 1520 DOI: 10.1039/C1CC16124E

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