Membrane        labeling and immobilization viacopper-free click chemistry

Heidi E. Bostic; Matthew D. Smith; Andrei A. Poloukhtine; Vladimir V. Popik; Michael D. Best

doi:10.1039/C1CC14415D

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Membrane labeling and immobilization viacopper-free click chemistry†‡

Heidi E. Bostic,^a Matthew D. Smith,^a Andrei A. Poloukhtine,^b Vladimir V. Popik^b and Michael D. Best*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, The University of Tennessee, Knoxville, TN, USA
E-mail: mdbest@utk.edu
Fax: +1-865-974-9332
Tel: +1-865-974-8658

^b Department of Chemistry, The University of Georgia, Athens, GA, USA

Abstract

Copper-free click chemistry was employed to derivatize membrane bilayers. This approach uses an azido-lipid conjugate presented on liposomes, which can be labeled in bioorthogonal fashion viacyclooctyne-tagged reagents. An immobilization-based approach using streptavidin-coated microplates was exploited to evaluate membrane derivatization.

This article is part of the themed collection: Emerging Investigators 2012

Download options Please wait...

Supplementary files

Supplementary information PDF (1021K)

Article information

DOI: https://doi.org/10.1039/C1CC14415D
Article type: Communication
Submitted: 20 Jul 2011
Accepted: 21 Sep 2011
First published: 03 Oct 2011

Download Citation

Chem. Commun., 2012,48, 1431-1433

Permissions

Request permissions

Membrane labeling and immobilization viacopper-free click chemistry

H. E. Bostic, M. D. Smith, A. A. Poloukhtine, V. V. Popik and M. D. Best, Chem. Commun., 2012, 48, 1431 DOI: 10.1039/C1CC14415D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications