Issue 9, 2012

Exploring the temperature-dependent kinetics and thermodynamics of immobilized glucose oxidase in microchip

Abstract

Herein, we report a method to investigate the thermodynamics and kinetics of immobilized enzyme (Glucose Oxidase, GOD) catalytic reaction on a microfluidics platform with precise temperature-control using a home-made plexiglass temperature controllable holder. This approach allows us to extract kinetic and thermodynamic parameters of the immobilized enzyme catalytic reactions easily, showing significant advantages over the traditional calorimetric and microcalorimetric methods which require complex fabrication of thermally isolated system. In our approach, the Arrhenius equation is introduced to establish the relationship between the kinetics and thermodynamics of the immobilized GOD. Results show that the obtained activation energy (Ea = 60.56 kJ mol−1) and the activation enthalpy (ΔHa = 53.08 kJ mol−1) are smaller than free enzymes, demonstrating that the immobilized GOD exhibits improved thermal stability compared with free enzymes. The present work offers an alternative approach to achieve the kinetics and thermodynamics of immobilized enzyme catalytic reactions on a microfluidics chip and promote our understanding of enzyme catalytic reactions.

Graphical abstract: Exploring the temperature-dependent kinetics and thermodynamics of immobilized glucose oxidase in microchip

Supplementary files

Article information

Article type
Paper
Submitted
11 May 2012
Accepted
08 Jun 2012
First published
13 Jun 2012

Anal. Methods, 2012,4, 2831-2837

Exploring the temperature-dependent kinetics and thermodynamics of immobilized glucose oxidase in microchip

L. Chen, Z. Wu, C. Wang, J. Ouyang and X. Xia, Anal. Methods, 2012, 4, 2831 DOI: 10.1039/C2AY25477H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements