A novel molecularly imprinted electrochemiluminescence sensor for isoniazid detection

Abstract

Based on Ru(bpy)₃²⁺-Au nanoparticles decorated multi-walled carbon nanotubes composites and a molecularly imprinted polymer (MIP), we propose a novel molecularly imprinted electrochemiluminescence (ECL) sensor to selectively determine isoniazid (INH). The MIP is synthesized through electrochemical copolymerization of acrylamide and N,N′-methylene diacrylamide in the presence of INH template molecules. The enhanced ECL intensity is linear in the range of 0.1 to 110 μg cm⁻³ and the detection limit is 0.08 μg cm⁻³ (3σ) INH with relative standard deviation 3.8% (n = 6) for 8 μg cm⁻³. As a result, the sensor has been successfully applied to the determination of INH in human urine and pharmaceutical samples. Moreover, the possible ECL mechanism is discussed.