Issue 3, 2011

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Abstract

A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a CogentĀ® Diamond Hydride column.

Graphical abstract: Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Article information

Article type
Technical Note
Submitted
17 Nov 2010
Accepted
01 Feb 2011
First published
24 Feb 2011

Anal. Methods, 2011,3, 742-744

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

G. K. Webster, A. Elliott, A. Dahan and J. M. Miller, Anal. Methods, 2011, 3, 742 DOI: 10.1039/C0AY00698J

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