Issue 3, 2011
From the journal:

Analytical Methods

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster,*a   Ashley Elliott,a   Arik Dahanb  and  Jonathan M. Millerc  

* Corresponding authors

a Analytical R&D, Abbott Laboratories, Abbott Park, IL, USA
E-mail: gregory.webster@abbott.com
Tel: +1 847 935-1295

b Department of Clinical Pharmacology, School of Pharmacy, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

c Global Formulation Sciences, Abbott Laboratories, Abbott Park, IL, USA

Abstract

A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

Graphical abstract: Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

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Article information

DOI
https://doi.org/10.1039/C0AY00698J
Article type
Technical Note
Submitted
17 Nov 2010
Accepted
01 Feb 2011
First published
24 Feb 2011

Anal. Methods, 2011,3, 742-744

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Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

G. K. Webster, A. Elliott, A. Dahan and J. M. Miller, Anal. Methods, 2011, 3, 742 DOI: 10.1039/C0AY00698J

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