Issue 3, 2011
From the journal:

Analytical Methods

UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: a simple, rapid and sensitive quantification method

David Touboul,*a   Joanna Wdzieczak-Bakala,a   Olivier Laprévoteab  and  Maria-Concepcion Garcia-Alvareza  

* Corresponding authors

a Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, Gif-sur-Yvette Cedex, France
E-mail: touboul@icsn.cnrs-gif.fr
Fax: +33 1 6907 7247
Tel: +33 1 6982 4588

b Laboratoire de Chimie-Toxicologie Analytique et cellulaire, EA4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 4 avenue de l'Observatoire, Paris, France
E-mail: olivier.laprevote@parisdescartes.fr

Abstract

Fast and reproducible quantification of thymosins β4 and β10 in different cell cultures was achieved by ultra high performance liquid chromatography coupled to mass spectrometry. We demonstrated that cancer cell lines all exhibit a higher amount of Tβ10 compared to control cells, whereas the level of Tβ4 is drastically depending on cell lines.

Graphical abstract: UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: a simple, rapid and sensitive quantification method

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Article information

DOI
https://doi.org/10.1039/C0AY00576B
Article type
Paper
Submitted
23 Sep 2010
Accepted
12 Jan 2011
First published
14 Feb 2011

Anal. Methods, 2011,3, 678-682

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UPLC-ESI-MS analysis of thymosins β4 and β10 in cell lysates: a simple, rapid and sensitive quantification method

D. Touboul, J. Wdzieczak-Bakala, O. Laprévote and M. Garcia-Alvarez, Anal. Methods, 2011, 3, 678 DOI: 10.1039/C0AY00576B

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