Issue 34, 2010
From the journal:

Physical Chemistry Chemical Physics

Reversible conformational change of cytochrome c at a modified goldelectrode in methanol

Suzanne Crillya  and  Edmond Magner*a  

* Corresponding authors

a Materials and Surface Science Institute, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
E-mail: Edmond.Magner@ul.ie
Fax: +353 61 203529
Tel: +353 61 202629

Abstract

Changes in the secondary structure of cytochrome c on immersion in methanol were monitored using circular dichroism. These changes occurred immediately in methanol, upon re-immersion in aqueous buffer, the secondary structure was restored in ca. 45 min. This method enables the secondary structure of proteins in non-aqueous solvents to be monitored in real time.

Graphical abstract: Reversible conformational change of cytochrome c at a modified gold electrode in methanol

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Article information

DOI
https://doi.org/10.1039/C0CP00350F
Article type
Paper
Submitted
26 Apr 2010
Accepted
14 Jul 2010
First published
03 Aug 2010

Phys. Chem. Chem. Phys., 2010,12, 10093-10097

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Reversible conformational change of cytochrome c at a modified gold electrode in methanol

S. Crilly and E. Magner, Phys. Chem. Chem. Phys., 2010, 12, 10093 DOI: 10.1039/C0CP00350F

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