Gas pressure effects on the rates of catalytic H2 oxidation by hydrogenases

James A. Cracknell; Bärbel Friedrich; Fraser A. Armstrong

doi:10.1039/C0CC03292A

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Gas pressure effects on the rates of catalytic H₂oxidation by hydrogenases†‡

James A. Cracknell,^a Bärbel Friedrich^b and Fraser A. Armstrong*^a

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* Corresponding authors

^a Department of Chemistry, Inorganic Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK
E-mail: fraser.armstrong@chem.ox.ac.uk
Fax: +44 (0)1865 272690
Tel: +44 (0)1865 272647

^b Institut für Biologie/Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestr. 117, 10115 Berlin, Germany
E-mail: baerbel.friedrich@rz.hu-berlin.de
Fax: +49 30 2093 8102
Tel: +49 30 2093 8101

Abstract

During catalysis by hydrogenases, entities no larger than H₂ or H⁺ reach and leave a deeply buried active site, by as yet unidentified pathways. Novel experiments, conducted mainly with the membrane-bound [NiFe]-hydrogenase from Ralstonia eutropha, explore why small excess gas pressures (H₂ or He) attenuate the rate of H₂ oxidation.

This article is part of the themed collection: Hydrogen

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Supplementary files

Determination of the change in H ₂ pressure and solution concentration as a function of the gas flow rate; the effect of varying potential, pH and temperature PDF (440K)

Article information

DOI: https://doi.org/10.1039/C0CC03292A
Article type: Communication
Submitted: 16 Aug 2010
Accepted: 09 Sep 2010
First published: 05 Oct 2010

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Chem. Commun., 2010,46, 8463-8465

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Gas pressure effects on the rates of catalytic H₂ oxidation by hydrogenases

J. A. Cracknell, B. Friedrich and F. A. Armstrong, Chem. Commun., 2010, 46, 8463 DOI: 10.1039/C0CC03292A

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