Issue 20, 2009
From the journal:

Soft Matter

Effect of mechanical properties of hydrogel nanoparticles on macrophage cell uptake†

Xavier Banquy,a   Fernando Suarez,b   Anteneh Argaw,c   Jean-Michel Rabanel,a   Peter Grutter,b   Jean-François Bouchard,c   Patrice Hildgenab  and  Suzanne Giasson*adef  

* Corresponding authors

a Faculty of Pharmacy, Université de Montréal, C.P. 6128, succursale Centre-Ville, Montréal, Québec, Canada

b Department of Physics, McGill University, 3600 University Street, Montréal, Québec, Canada

c Ecole d'Optométrie, Université de Montréal, C.P. 6128, succursale Centre-Ville, Montréal, Québec, Canada

d Groupe de Recherche Universitaire sur le Médicament de l'Université de Montréal, C.P. 6128, succursale Centre-Ville, Montréal, Québec, Canada

e Department of Chemistry, Université de Montréal, C.P. 6128, succursale Centre-Ville, Montréal, Québec, Canada
E-mail: Suzanne.giasson@umontreal.ca
Fax: +01 514 340 5290
Tel: +01 514 340 5175

f Center for Self-Assembled Chemical Structures, McGill University, 801 Sherbrooke St. West, Montréal, Québec, Canada

Abstract

Uptake and intracellular trafficking of hydrogel nanoparticles (NPs) of N,N-diethyl acrylamide and 2-hydroxyethyl methacrylate crosslinked with N,N′-methylene-bis-acrylamide were studied with a RAW 264.7 murine macrophage cell line. Results show that the uptake rate, the mechanism of internalization and the concentration of internalized NPs are correlated to the NP Young modulus. Soft NPs are found to be internalized preferentially via macropinocytosis while the uptake of stiff NPs is mediated by a clathrin-dependent mechanism. NPs with an intermediate Young modulus exhibit multiple uptake mechanisms. The accumulation rate of the NPs into lysosomal compartments of the cell is also dependent on the NP elasticity. Our results suggest that control over the mechanical properties of hydrogel NPs can be used to tailor the cellular uptake mechanism and kinetics of drug delivery.

Graphical abstract: Effect of mechanical properties of hydrogel nanoparticles on macrophage cell uptake

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Article information

DOI
https://doi.org/10.1039/B821583A
Article type
Paper
Submitted
02 Dec 2008
Accepted
30 Jun 2009
First published
03 Aug 2009

Soft Matter, 2009,5, 3984-3991

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Effect of mechanical properties of hydrogel nanoparticles on macrophage cell uptake

X. Banquy, F. Suarez, A. Argaw, J. Rabanel, P. Grutter, J. Bouchard, P. Hildgen and S. Giasson, Soft Matter, 2009, 5, 3984 DOI: 10.1039/B821583A

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