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A new “dual-mode” chromogenic and fluorescent turn-on probe (2) for the selective sensing of biological thiols is reported. In MeOH–H2O cosolvent at physiological pH 7.40 (MeOH–H2O = 3:7), biological thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the chromo- and fluorophore merocyanine (3).

Graphical abstract: A colorimetric and fluorescent merocyanine-based probe for biological thiols

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