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Abstract | Cited by

Herein we report a feasibility study for a new concept to detect DNA binding protein NF-κB based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.

Graphical abstract: A new assay format for NF-κB based on a DNA triple helix and a fluorescence resonance energy transfer

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