Yongwook
Kim
a,
Nam Woong
Song
*bc,
Hyunung
Yu
b,
Dae Won
Moon
b,
Sung Jun
Lim
a,
Wonjung
Kim
a,
Hye-Joo
Yoon
a and
Seung
Koo Shin
*a
aBionanotechnology Center, Department of Chemistry, Pohang University of Science and Technology, San31 Hyoja-dong Nam-gu Pohang, Kyungbuk, 790-784, Korea. E-mail: skshin@postech.ac.kr; Fax: +82 (0)54 2793399; Tel: +82 (0)54 279
2123
bNanobio Fusion Research Center, Korea Research Institute of Standards and Science, 1 Doryong-dongYuseong-gu, Daejeon, 305-340, Korea. E-mail: nwsong@kriss.re.kr; Fax: +82 (0)42 8685032; Tel: +82 (0)42 868
5214
cCell Dynamics Research Center, Gwangju Institute of Science and Technology, 261 Oryong-dong Buk-gu, Gwangju, 500-712, Korea
First published on 3rd March 2009
Blinking of zinc-blende CdSe/ZnS core/shell nanocrystals are studied as a function of surface passivating ligands. Organic-soluble CdSe/ZnS core/shell nanocrystals are prepared by colloidal synthesis free of trioctylphosphine oxide and converted into water-soluble ones by ligand exchange with three different hydrophilic thiols, 2-aminoethanethiol, 3-mercapto-1-propanol, and 3-mercaptopropionic acid. The zinc-blende lattice structure is confirmed by powder X-ray diffraction, the size and shape distributions are visualized by high-resolution transmission electron microscopy, and hydrodynamic size distributions of water-soluble nanocrystals are determined by dynamic light scattering. Ligand-dependent optical properties, such as the absorption and emission spectra as well as the photoluminescence lifetime, are obtained in both solution and glass substrate to characterize the effects of ligand on the bright state of nanocrystals. The time trace of blinking is recorded for single nanocrystals in polymer film. Both on- and off-time distributions are fit to a power law. The off-time exponents are clustered at 1.67 ± 0.05, whereas the on-time exponents are scattered in the range of 1.71–2.25. The thiolate conjugation on the surface zinc atom greatly reduces the on-time duration, suggesting that the rate of photoinduced charge separation from the bright (on) to the dark (off) state increases as the number of surface hole-trap states increases.
Although many types of single fluorophores, such as organic dyes, fluorescent proteins and conjugated polymers, are known to emit light intermittently,3,4 semiconductor nanocrystals exhibit by far the most long-lasting blinking phenomena that follow a simple power law,11p(t) ∝t−α, for both on- and off-time probability distributions.11–13 The power-law statistics holds over a wide range of timescale from microseconds to seconds regardless of nanocrystal composition, shape, and size under various temperatures and excitation fluences.14–17 To explain seemingly universal power-law behaviors, a number of models have been proposed to date.14–21 A general consensus is that blinking is a random switching between the neutral (on) and charged (off) states.2–4 For instance, the photoinduced ejection of an electron from the core of nanocrystal to the surrounding can leave a hole behind, thus creating a charge-separated state that efficiently quenches emission. However, the predicted power-law exponents vary with blinking models.2–4,14–21 The key difference among proposed models lies in the mobility of the separated charges.3 Trap models assume tunneling of the photoexcited electron between the emitting and immobile trap states,14,15 whereas diffusion models assume random walk of the ejected electron around the ionized nanocrystal.16,17 A more elaborate diffusion-controlled electron transfer18–20 or Anderson localization model21 extends the nature and dynamics of the trap or localized state. To unravel the mechanism of power-law blinking, we need to characterize the charge-separated states under well-controlled conditions. Such an attempt has been recently made by Cichos and co-workers by varying the dielectric media surrounding CdSe/ZnS nanocrystals.15 They have found an interesting correlation of the off-time exponent with the dielectric constant and proposed self-trapped states of the ejected electron in dielectric media as a source of power-law blinking.
Of the semiconductor nanocrystals, the CdSe/ZnS core/shell nanocrystal is one of the most extensively studied systems in blinking. Most of them are based on the wurtzite (W) CdSe nanocrystal.11–14,16,20,22 However, blinking studies on the zinc-blende (ZB) CdSe/ZnS nanocrystal have rarely been reported. We have previously shown that ZB CdSe-based nanocrystals have an advantage over W nanocrystals in that the shell can be grown more uniformly over the core and water-soluble ZB nanocrystals are highly luminescent (∼50% PL efficiency) with the least amount of shell thickness.23 We have also characterized the ligand coordinated on the surface of ZB CdSe/ZnS nanocrystals by time-of-flight (TOF) secondary ion mass spectrometry (SIMS).24 TOF-SIMS imaging has shown that organic-soluble nanocrystals are covered with stearate, whereas water-soluble ones are coated with thiolate via zinc-thiolate linkage. Thus our well-characterized ZB CdSe/ZnS nanocrystals allow variations in density of hole-trap states and offer a new opportunity to study the effects of surface ligand on blinking of single nanocrystals. Herein, we present evidence of hole-trap states mediating the on-time probability distribution.
We employ both organic- and water-soluble ZB CdSe/ZnS nanocrystals, obtain the absorption and emission spectra as well as PL lifetimes to characterize the effects of surface ligand on the bright emitting state of nanocrystals in solution and glass substrate, and monitor the blinking of single nanocrystals in polymer film.
![]() | ||
Scheme 1 Preparation of organic-soluble (ORG) CdSe/ZnS nanocrystals and water-soluble CdSe/ZnS nanocrystals by ligand exchange with 2-aminoethanethiol (AET), 3-mercaptopropanol (MPO), and 3-mercaptopropinic acid (MPA). |
The output from a 543.5 nm HeNe laser was coupled to a single-mode fiber, filtered at the end, and directed to a Carl Zeiss Fluar 100× oil-immersion objective. At the sample spot, a Gaussian laser beam was ∼300 nm in diameter, and the laser fluence of 2.8 and 28 kW cm−2 was used for blinking and spectrum acquisition, respectively. The emitted light was collected with the same objective and focused onto a detector. A Dongwoo Optron 150-mm spectrograph (300 gr mm−1) equipped with a Princeton Instruments 1340 × 100 TE/CCD was used to take the PL spectra. A PerkinElmer APD photon counting module SPCM-AQR-13-FC was used to take the image and blinking. A 100 × 100 pixel image of single nanocrystals was obtained over a 20 × 20 μm area by rastering a sample on a Physik Instrumente P-611.3S nanopositioning system with 10 ms duration at each pixel. A time trace of blinking was monitored for 10–20 s with 1 ms bin by positioning the laser beam over a bright spot. About 50–100 single spots were tested from 4–5 different areas.
![]() | ||
Fig. 1 Powder X-ray diffraction patterns of CdSe and CdSe/ZnS nanocrystals. The inset shows an HRTEM image of a single nanocrystal. Vertical lines represent the diffraction patterns for bulk zinc-blende (ZB) compounds. |
The hydrodynamic size distributions of WS-NCs are shown in Fig. S2 of the ESI.† The MPA-capped CdSe nanocrystal is also compared with CdSe/ZnS WS-NCs. The average hydrodynamic size is 10.0 ± 2.0, 10.2 ± 2.4, 12.7 ± 3.0 nm for AET-, MPO-, and MPA-capped CdSe/ZnS, respectively, and 9.4 ± 1.6 nm for MPA-capped CdSe. Yamamoto and co-workers have reported a broad size distribution around 40 nm for AET-capped CdSe/ZnS synthesized in trioctylphosphine oxide (TOPO).26 By contrast, our TOPO-free nanocrystals yield a narrow size distribution with no indication of clusters greater than 20 nm. In TOPO-based nanocrystals, the impurities present in TOPO, such as phosphinic acids, may coordinate strongly onto the surface, survive the ligand exchange step and facilitate the cluster formation via electrostatic interactions with the terminal ammonium groups on adjacent nanocrystals. MPA-capped nanocrystals appear to be larger than others. A difference of 3.3 nm between the two MPA-capped CdSe/ZnS and CdSe nanocrystals is greater than the thickness of the ZnS shell (6 × 3.1 Å).
Both the absorption and emission spectra are presented in Fig. 2. The 1S3/21Se absorption band appears at 596 and 606 nm for CdSe and CdSe/ZnS ORG-NCs in chloroform, respectively, and 600, 596 and 600 nm for AET-, MPO- and MPA-capped WS-NCs, respectively. The ZnS shell passivation induces a 34-meV redshift in chloroform, while the zinc-thiolate linkage on the ZnS shell leads to a 20–34-meV blueshift in water. The solvatochromatic effect is supposed to induce a redshift in absorption rather than a blueshift as the solvent dielectric constant (ε) increases from 4.8 (chloroform) to 78 (water).27 The observed spectral shifts indicate the perturbation of the CdSe core electronic structure by surface passivation. TOF-SIMS imaging has shown that ORG-NCs are covered with weakly-coordinating stearates, while WS-NCs are covered with covalently-coordinating thiolate ligands.24 The surface dangling bonds on the ZnS shell are different in the two types of nanocrystals. Stearate-covered ORG-NCs face the full extent of surface reconstruction due to the dangling bonds, which could lead to the lattice distortion of the CdSe core that induces a redshift in absorption.23 On the other hand, thiolate-covered WS-NCs face such a distortion to a lesser degree because the zinc-thiolate linkage effectively uses up the dangling bonds of the surface zinc atoms, which could turn the spectral shift back to normal.
![]() | ||
Fig. 2 (a) Absorption (thin line) and emission (thick line) spectra of CdSe and CdSe/ZnS nanocrystals soluble in chloroform and of AET-, MPO-, and MPA-capped CdSe/ZnS nanocrystals soluble in water. The dashed line denotes the emission spectra of nanocrystal aggregates on a glass substrate; (b) (top) PL decay of organic-soluble (ORG) CdSe/ZnS nanocrystals in chloroform and AET-, MPO-, and MPA-capped CdSe/ZnS nanocrystals in water; (bottom) PL decay of organic- and water-soluble CdSe/ZnS nanocrystal aggregates on a glass substrate. |
The emission spectra of nanocrystals in solution (Fig. 2) show the variation of the Stokes shift between the absorption peak and the 1Se1S3/2 emission maximum depending on the type of surface ligands. The PL maximum appears at 611 and 621 nm for CdSe and CdSe/ZnS ORG-NCs in chloroform, respectively, but with almost identical Stokes shift of 49–51 meV. WS-NCs yield the PL maximum at 615–618 nm with Stokes shift of 57–61 meV. Values of full width at half maximum are listed in Table 1 along with other optical properties. The Stokes shift of WS-NCs is 8–12 meV greater than that of ORG-NCs.
Nanocrystals in solutiona | Aggregates on glass substrateb | Single nanocrystals in polymer filmc | ||||
---|---|---|---|---|---|---|
Type of surface ligands | λ abs d/nm | λ em e/nm | τ eff f/ns | λ em e/nm | τ eff f/ns | 〈λem〉g/nm |
a ORG-NCs were in chloroform and WS-NCs were in distilled water. b Nanocrystal aggregates were formed by drying a drop of nanocrystal solution on glass substrate. c ORG-NCs were in PMMA, while WS-NCs were in PVA. d The band edge absorption peak for the 1S3/21Se electronic transition. e The emission maximum (full width at half maximum in parenthesis). f The effective lifetime calculated from the multiple-component PL decay curve. g The average value of emission maximum from 50 different single NCs. | ||||||
ORG | 606 | 621 (36) | 6.7 | 622 (36) | 9.8 | 614 |
AET | 600 | 618 (34) | 10.8 | 627 (37) | 10.7 | 618 |
MPO | 596 | 615 (35) | 8.1 | 626 (39) | 8.9 | 620 |
MPA | 600 | 617 (35) | 8.9 | 628 (38) | 10.4 | 617 |
The emission spectra of nanocrystal aggregates on a glass substrate (dotted lines in Fig. 2) are red-shifted by different degrees depending on the surface ligand. Stearate-covered ORG-NCs show little shift in emission from 621 to 622 nm, whereas thiolate-covered WS-NCs yield 26–36 meV redshift from 615–618 to 626–628 nm. The two types of ligands differ in their alkyl chain lengths. Stearate has a C18-alkyl chain, but thiolates have C2–C3 alkyl chains. To check the effects of alkyl chain length on the spectral shift in aggregates, we replaced C3-alkyl MPO with C11-alkyl mercaptoundecanol (MUO). MUO-capped NCs result in only a small redshift in emission upon transition from solution to aggregates (data not shown). Thus, the observed redshift in emission from WS-NCs covered with short-chain thiolates is very likely due to the Förster resonance energy transfer (FRET) between different size nanocrystals within the aggregates. Because the FRET efficiency is inversely proportional to the sixth power of the distance between the donor and acceptor fluorophores, the amount of spectral shift should decrease with increasing chain length of the surface ligand. Provided that nanocrystals form close-packed but amorphous aggregates on a glass substrate as the solvent evaporates slowly,28 the inter-particle distance in WS-NC aggregates might be 3–4 nm shorter than the distance in ORG-NC aggregates, which could lead to the redshift in emission.
![]() | ||
Fig. 3 (a) Image of single organic-soluble CdSe/ZnS nanocrystals in PMMA film; (b) the ensemble average spectra of organic- and water-soluble CdSe/ZnS nanocrystals and the statistical distribution of the PL maximum. |
The blinking time trace of ORG-NCs in PMMA is shown in Fig. 4a along with an occurrence histogram on the right. A threshold separating “on” from “off” state in a bimodal distribution is set at three times the standard deviation of the average dark count. Both on- and off-time distributions are presented in Fig. 4b and 4c, respectively, and they are fit to a power law of the form:
P(t) = At−α |
![]() | ||
Fig. 4 (a) A blinking time trace of a single organic-soluble CdSe/ZnS nanocrystal and a histogram of blinking; (b) the on-time probability distributions and (c) the off-time probability distributions of organic- and water-soluble CdSe/ZnS nanocrystals. The lines denote the least-square fit to a power law. |
In the present experiment, both ORG- and WS-NCs are derived from the same batch of TOPO-free colloidal synthesis, so that the only difference lies in the surface ligands. Moreover, the surface ligands and optical properties of both ORG- and WS-NCs are well characterized. In ensemble measurements, the spectral features of WS-NCs are almost identical to that of ORG-NC, and the PL lifetimes of WS-NCs are of the same order of magnitude as that of ORG-NC. However, the blinking of single nanocrystals presents remarkable ligand-dependent phenomena. The on-state durations strongly depend on surface ligand, as noted by others.10,20,30 Apparently, the zinc-thiolate linkage in WS-NCs reduces the on-state durations more than the stearate and/or PMMA carbonyl coordination in ORG-NCs does. Thus we can draw a meaningful correlation between the mode of surface conjugation and blinking. The zinc-thiolate linkage forms a coordinate covalent bond, whereas stearate and/or PMMA carbonyls noncovalently coordinate onto the surface zinc atom. The zinc-thiolate linkage effectively uses up the surface dangling bonds of the zinc atom, which removes the electron-trap states, but at the same time the two nonbonding electron pairs of the sulfur atom become the hole-trap states. To the contrary, the stearate and/or PMMA carbonyl coordination perturbs the energy levels of the band structure, but they do not remove or add any new trap states. As a result, the zinc-thiolate linkage on WS-NCs, which increases the density of hole-trap states, ends up suppressing the on-state durations. This interpretation is in accord with a report supposing that thiolate deactivates electron-trap states at low concentrtions and introduces new hole-trap states at high concentrations.31 It is worth mentioning the previous finding of near-complete suppression of blinking in streptavidin-coated CdSe/ZnS nanocrystals by β-mercaptoethanol in pH 7.4 buffer.32 Because β-mercaptoethanol hardly dissociates to thiolate at neutral pH,31 we think their suppression of blinking is not due to thiolates. In our system, hydrophilic thiols are shifted to thiolates by equilibrium under basic conditions. Thus, the thiolates conjugated onto the surface zinc atom are the cause of on-time variation.
Footnote |
† Electronic supplementary information (ESI) available: High-resolution transmission electron microscope images, hydrodynamic size distributions obtained from dynamic light scattering and variation profiles of photoluminescence decay components. See DOI: 10.1039/b822351c |
This journal is © the Owner Societies 2009 |