Issue 12, 2006
From the journal:

Photochemical & Photobiological Sciences

Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors†

Hendrik Naumann,a   Johann P. Klare,b   Martin Engelhard,c   Peter Hildebrandta  and  Daniel H. Murgida*a  

* Corresponding authors

a Technische Universität Berlin, Institut für Chemie, Max Volmer Laboratorium für Biophysikalische Chemie, Sekr. PC 14, Straße des 17. Juni 135, Berlin, Germany
E-mail: dh.murgida@tu-berlin.de
Fax: +49 30 314 21122
Tel: +49 30 314 26500

b Universität Osnabrück, Fachbereich Phyisk, Barbarastr. 7, Osnabrück, Germany

c Max-Planck-Institut für Molekulare Physiologie, Otto-Hahn-Straße 11, Dortmund, Germany

Abstract

This article describes a method, based on surface-enhanced resonance Raman (SERR) spectroscopy, for studying the reaction dynamics of photoreceptors immobilized on metal electrodes. Time resolution and fresh sample conditions are achieved by synchronizing the rotational and translational motion of a novel kinematic electrode with the duration and time delay between the pump and probe events. The power and sensitivity of the method is illustrated by studying the photocycle of the sensory photoreceptor NpSRII and its sensitivity to the applied electric field. The results are compared with time-resolved resonance Raman measurements in solution.

Graphical abstract: Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors

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Article information

DOI
https://doi.org/10.1039/B607807A
Article type
Perspective
Submitted
01 Jun 2006
Accepted
22 Sep 2006
First published
17 Oct 2006

Photochem. Photobiol. Sci., 2006,5, 1103-1108

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Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors

H. Naumann, J. P. Klare, M. Engelhard, P. Hildebrandt and D. H. Murgida, Photochem. Photobiol. Sci., 2006, 5, 1103 DOI: 10.1039/B607807A

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