Steven J.
Moss‡
a,
Isabelle
Carletti‡
a,
Carlos
Olano
b,
Rose M.
Sheridan
a,
Michael
Ward
a,
Vidya
Math
a,
Mohammad
Nur-E-Alam
a,
Alfredo F.
Braña
b,
Ming Qiang
Zhang
a,
Peter F.
Leadlay
c,
Carmen
Méndez
b,
José A.
Salas
b and
Barrie
Wilkinson
*a
aBiotica, Chesterford Research Park, Little Chesterford, UK CB10 1XL. E-mail: barrie.wilkinson@biotica.com; Fax: +44 (0)1799 532920; Tel: +44 (0)1799 532925
bDepartamento de Biologia Funciona e Instituto Universitario de Oncologia del Principado de Asturias (I.U.O.P.A.), Universidad de Oviedo, 33006 Oviedo, Spain
cDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, UK CB2 1GA
First published on 11th May 2006
We report the directed biosynthesis of borrelidin analogues and their selective anti-proliferative activity against human cancer cell lines.
As part of our anti-cancer drug discovery programmes 1 was selected as a lead molecule. We previously cloned and sequenced the gene cluster from Streptomyces parvulus Tü4055 that governs the biosynthesis of 1.4 This allowed us to propose a biosynthetic pathway in which a modular polyketide synthase (PKS) generates the macrolactone structure using a molecule of cyclopentane-trans-1,2-dicarboxylic acid 2 as a starter unit to initiate polyketide chain synthesis (see Figs. 1 and 2 for structures). The only post-PKS modification involves the unusual conversion of the methyl group at C12 of the first PKS-free intermediate (pre-borrelidin, 3) into a nitrile moiety.5 The absolute configuration of 2 as the 1R,2R isomer follows from the known absolute structure of 1.6 We now report the rational biosynthetic engineering of the bor gene cluster of S. parvulus Tü4055 in order to produce novel analogues of 1.
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Fig. 1 Chemical structures of borrelidin 1 and pre-borrelidin 3. |
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Fig. 2 Precursor directed biosynthesis of 1 from 2 using S. parvulus mutant BIOT-1302. |
In previous work we identified S. parvulus Tü4055 mutants which did not produce 1, or any analogues of 1, unless 2 was added (Fig. 2).4 These mutants therefore allow analogues of 2 to be tested as starter units in order to produce novel borrelidin analogues by mutasynthesis. This approach has previously been used with success to produce analogues of several therapeutically important polyketides7 including the mTOR inhibitor rapamycin8 and the antihelmintic avermectin.9 For the experiments described here we utilised a S. parvulus mutant disrupted in the gene borG (BIOT-1302).4 Mutants deleted in other bor genes could also be restored to borrelidin production by added 2, however when supplemented with 2 BIOT-1302 unexpectedly produced 1 at levels between 15- and 20-fold higher than the parent strain under identical conditions, and around 5-fold higher titre than the parent strain supplemented with equivalent levels of 2. Although we cannot yet assign a definite function to the borG product BorG, we speculate it may be involved in starter unit biosynthesis or in the regulation of borrelidin biosynthesis.
More than 40 mono- and dicarboxylic acids were fed to BIOT-1302 cultures (see supplementary information for full details). All of the mono- and most of the dicarboxylic acids examined failed to initiate the biosynthesis of novel analogues of 1. Indeed, of the several cyclic dicarboxylic acids examined, only cyclobutane-trans-1,2-dicarboxylic acid produced a novel compound when fed to BIOT-1302; this was produced at ∼ 50% of the titre of 1 obtained when 2 was fed. Fermentation was then scaled up and after purification the new compound was shown to have the expected structure, 4 (Fig. 3; see supplementary information for NMR and MS details). The cis-isomer (meso form) of cyclobutane-1,2-dicarboxylic acid failed to act as a PKS starter unit, as did the cis-cyclohexane and trans-cyclohexane analogues, the cis-cyclopentane and cyclopent-1-ene-1,2-dicarboxylic acids, and the trans-cyclopropane analogue. Assuming that all of these analogues are converted by a CoA ligase to activated CoA monoesters inside the cell, these results suggest that the PKS loading module recognises a rather specific geometrical arrangement of the adjacent carboxylate groups. It is consistent with this idea that, although succinic acid itself was not a substrate, the 2-methyl- and 2,3-dimethylsuccinic acids were accepted and novel compounds were produced. 2,3-Dimethylsuccinic acid is commercially available either in the meso form, or as a mixture of racemic and meso forms. When fed the mixture BIOT-1302 produced only a (single) novel compound. This was expected based on the stereochemistry of 2. The new compound was produced at ∼15% of the titre of 1 obtained when 2 was fed. The compound was isolated from a larger-scale fermentation and its structure was confirmed as 5. When fed the meso form only no products were produced. When racemic 2-methylsuccinic acid was fed three new compounds were produced, with the major compound 6 produced at approximately half of the titre for 5 above. After scale-up of fermentation and isolation, the three compounds were identified as 6–8 respectively. The major product 6 has a methyl branch adjacent to the carboxylic acid moiety utilised for polyketide biosynthesis. No other α-methyl regioisomer was obtained and the absolute structure of 6 is based on that of 1 and on the results of further feeding experiments described below. 7 and 8 derive from the incorporation of 2-methylsuccinic acid with the alternative regiochemistry, i.e. where incorporation by the PKS gives a β-methyl substitution relative to the carboxylic acid moiety used for polyketide chain extension. Apparently, both isomers of the fed substrate are regiospecifically incorporated. The stereochemistry at the β-methyl for each of these was assigned based on the experiments described below.
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Fig. 3 Novel borrelidin analogues generated by precursor directed biosynthesis. |
To verify that 6 was indeed derived from the (2R)-isomer of 2-methylsuccinic acid, the (2R)- and (2S)-2-methylsuccinic acids were fed separately to BIOT-1302. As expected, feeding the (2R)-isomer produced a major compound 6 and a minor compound 7, but no 8. When the (2S)-isomer was fed only 8 was produced.
In addition to feeding analogues of the PKS starter unit as their free acids, we examined the effect of adding substituted succinates as their anhydrides (readily available and cheap) to the fermentation. In fact, similar titres were obtained as for the parent acids. We also fed itaconic anhydride, which gave the same product profile as (2R)-2-methylsuccinic acid, demonstrating that itaconic anhydride is reduced to (2R)-2-methylsuccinic anhydride, or its free acid, prior to incorporation by the loading module of the PKS. We then fed racemic 2-(mercaptoacetyl)succinic acid anhydride to cultures of BIOT-1302 and observed the production of a new compound, albeit at significantly lower titre than in our other experiments. Larger-scale fermentation and isolation allowed its identity to be established as the 2-thiomethyl substituted compound 9. It appears that in vivo 2-(mercaptoacetyl)succinic acid anhydride or a macrocyclic precursor of 9 undergoes an S-deacetylation followed by S-methylation; we predict that the timing of this will prove to be prior to utilisation by the PKS.
We examined the cytotoxicity of these novel borrelidin analogues against a number of cancer cell lines in vitro (MCF-7, MDA-MB-231, MDA-MB-468, NCI-H460, SF-268, OVCAR-3, A498, GXF 251L, MEXF 394NL, UXF 1138L, LNCAP and DU145;10 see supplementary information for experimental details). None of 4–9 were as potent as 1 in inhibiting the mean proliferation of these cancer cell lines as judged by mean growth inhibition of treated cells versus untreated (%T/C) (Table 1). However, the compounds showed interesting selectivity against certain cell lines. For example, 4 and 6 are equipotent to 1 against mammary MCF-7 and uterus 1138L although both are less active than 1 against other cell lines. Further analysis of these and anti-angiogenic evaluations are ongoing.
Compound | Mean %T/C | n | |
---|---|---|---|
at 1 µM | at 10 µM | ||
Borrelidin, 1 | 7 | 6 | 2 |
4 | 33 | 8 | 2 |
5 | 21 | 7 | 3 |
6 | 73 | 22 | 3 |
7 | 83 | 28 | 3 |
8 | 96 | 74 | 3 |
9 | 94 | 62 | 3 |
We have demonstrated that structural changes can be readily introduced into the portion of borrelidin derived from the starter unit by using mutasynthesis and, notably, that a mutant strain (BIOT-1302) of S. parvulus Tü4055 which carries a disrupted borG gene can be used to generate greatly improved titres of both 1 and these novel compounds.
This research was supported by grants from the Spanish Ministry of Education and Science (BMC2003-00478) and from the Plan Regional de Investigación del Principado de Asturias (GE-MED01-05); and by a project grant from the Biotechnology and Biological Sciences Research Council (B.B.S.R.C.) (8/B18119). We thank Obra Social Cajastur for financial support to C.O. We thank Oncotest GmbH (Freiburg, Germany) for carrying out the cancer cell line proliferation inhibition assays.
Footnotes |
† Electronic supplementary information (ESI) available: Fermentation, compound isolation and structure elucidation; cancer cell line assay procedure and individual data. See DOI: 10.1039/b602931k |
‡ These authors contributed equally. |
This journal is © The Royal Society of Chemistry 2006 |