Photosensitized NADH formation system with multilayer TiO2 film

Takashi Sagawa; Ryota Sueyoshi; Mikako Kawaguchi; Mayu Kudo; Hirotaka Ihara; Katsutoshi Ohkubo

doi:10.1039/B315382G

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Photosensitized NADH formation system with multilayer TiO₂ film†

Takashi Sagawa,*^a Ryota Sueyoshi,^b Mikako Kawaguchi,^a Mayu Kudo,^a Hirotaka Ihara^b and Katsutoshi Ohkubo*^a

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* Corresponding authors

^a Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan
E-mail: t-sagawa@iae.kyoto-u.ac.jp
Fax: +81 774 38 3516
Tel: +81 774 38 3511

^b Department of Applied Chemistry & Biochemistry, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan
E-mail: ihara@kumamoto-u.ac.jp
Fax: +81 96 342 3662
Tel: +81 96 342 3661

Abstract

A TiO₂/polymer film on a quartz plate fabricated by a layer-by-layer method was employed for NADH production from NAD⁺ with lipoamide dehydrogenase and methyl viologen in Tris-HCl buffer on irradiation with UV light, and the ultra thin film prevented from enzyme deactivating effectively.

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Supplementary files

Experimental and spectroscopic data DOC (793K)

Article information

DOI: https://doi.org/10.1039/B315382G
Article type: Communication
Submitted: 27 Nov 2003
Accepted: 19 Jan 2004
First published: 23 Feb 2004

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Chem. Commun., 2004, 814-815

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Photosensitized NADH formation system with multilayer TiO₂ film

T. Sagawa, R. Sueyoshi, M. Kawaguchi, M. Kudo, H. Ihara and K. Ohkubo, Chem. Commun., 2004, 814 DOI: 10.1039/B315382G

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Chemical Communications