Synthesising protease-stable isopeptides by proteases: an efficient biocatalytic approach on the basis of a new type of substrate mimeticsElectronic supplementary information (ESI) available: complete details for experimental procedures. See http://www.rsc.org/suppdata/cc/b1/b105752a/

Abstract

A biocatalytic route to the ‘post-synthesis’ formation of Asp/Glu-derived isopeptides is illustrated on the basis of the Staphylococcus aureus V8 protease used as the biocatalyst, a new type of substrate mimetics as the donor peptides, and several acceptor peptides varying in length and sequence.