Issue 5, 2001

Bar coding objects with DNAElectronic Supplementary Information available. See http://www.rsc.org/suppdata/an/b1/b101850g/

Abstract

A bioanalytical scheme is described for bar coding objects with DNA. Several DNA templates of different lengths are mixed together and the mixture used to label the object of interest. To disclose the bar code, the DNA mixture is eluted from the object, amplified by the polymerase chain reaction with a universal pair of primers, and then the amplified templates resolved by agarose gel electrophoresis. In its present state, the bar coding scheme allows for over a million different labels. The reliability of the scheme has been demonstrated in a typical situation by labelling a letter with a template mixture (∼1.5 fmol of DNA), sending the letter through the post and successfully reading the bar code on receipt of the letter.

Supplementary files

Article information

Article type
Communication
Submitted
27 Feb 2001
Accepted
20 Mar 2001
First published
04 Apr 2001

Analyst, 2001,126, 545-547

Bar coding objects with DNA

J. P. L. Cox, Analyst, 2001, 126, 545 DOI: 10.1039/B101850G

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