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DOI: 10.1039/A904504J (Paper) Reference Section for: J. Chem. Soc., Perkin Trans. 2, 1999, 1859-1863

Subtle differences in molecular recognition between modified glycopeptide antibiotics and bacterial receptor peptides identified by electrospray ionization mass spectrometry

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Thomas J. D. Jørgensen, Thomas Staroske, Peter Roepstorff, Dudley H. Williams and Albert J. R. Heck

Abstract

In determining structure–activity relationships, it is advantageous if binding constants for a variety of ligands to a given target molecule can be directly obtained from a single aqueous solution containing a mixture of ligands and the target molecule. In this paper further evidence is provided showing that electrospray ionization mass spectrometry (ESI-MS) can be used in the rapid quantitative analysis of mixtures of vancomycin-group antibiotics and their bacterial cell-wall receptors allowing the identification of even subtle differences in binding constants. Differences in affinities are quantified for a mixture of vancomycin antibiotics (vancomycin, dechlorovancomycin and N-demethylvancomycin) and for a mixture of ristocetin A and its pseudoaglycone. Binding constants determined by ESI-MS were found to be in close agreement with those determined by more direct methods in aqueous solution.

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