Layer-by-layer deposition of avidin and biotin-labeled antibody on a solid surface to prepare a multilayer array of antibody

Abstract

Multilayer thin films containing an antibody have been prepared successfully by depositing avidin and biotin-labeled antibody alternately and repeatedly on the surface of a quartz slide, in which the antibody retains its binding activity.

References

1. A. Riklin and I. Willner, Anal. Chem., 1995, 67, 4118.
2. Z. Chen, D. L. Kaplan, H. Gao, J. Kumar, K. A. Marx and S. K. Tripathy, Mater. Sci. Eng. C, 1996, 4, 155.
3. Y. Lvov, K. Ariga, I. Ichinose and T. Kunitake, J. Am. Chem. Soc., 1995, 117, 6117.
4. C. Bourdillon, C. Demaille, J. Moiroux and J.-M. Savéant, Acc. Chem. Rev., 1996, 29, 529.
5. J. Anzai, H. Takeshita, Y. Kobayashi, T. Osa and T. Hoshi, Anal. Chem., 1998, 70, 811.
6. J. Anzai, Y. Kobayashi and N. Nakamura, J. Chem. Soc., Perkin Trans. 2, 1998, 461.
7. F. Caruso, K. Niikura, D. Neil Furlong and Y. Okahata, Langmuir, 1997, 13, 3433.
8. E. Brynda, M. Houska, J. Skvor and J. J. Ramsden, Biosens. Bioelectron., 1998, 13, 165.
9. N. M. Green, Biochem. J., 1996, 101, 774; M. Wilchek and E. A. Bayer, Anal. Biochem., 1988, 171, 1.