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DOI: 10.1039/A901751H (Paper) Reference Section for: J. Anal. At. Spectrom., 1999, 14, 1505-1510

Accurate determination of iron, copper and zinc in human serum by isotope dilution analysis using double focusing ICP-MS

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Cristina Sariego Muñiz, Juan M. Marchante Gayón, J. Ignacio García Alonso and Alfredo Sanz-Medel

Abstract

The development of an ICP-MS method for the determination of three essential trace elements, Fe, Cu and Zn, in human serum by isotope dilution analysis is described. A double focusing ICP mass spectrometer (ELEMENT) working at a nominal resolution of 3000 allowed the complete resolution of polyatomic ions overlapping the main isotopes of these elements. The isotope ratios measured for the determination of Fe, Cu and Zn were 57Fe/56Fe, 65Cu/63Cu and 67Zn/64Zn, respectively. Instrumental parameters of the mass spectrometer were optimised for optimum precision, while keeping the total time of analysis under 6 min (2 min per element). The RSD values obtained for the isotope ratios of Fe (57/56), Cu (65/63) and Zn (67/64) were 0.7, 0.2 and 0.8%, respectively, for a natural standard aqueous solution of 50 µg l–1. The measured isotope ratios were corrected for dead time on the detector and mass discrimination errors. The sample to spike ratio was optimised to minimise the relative error on the isotope ratio measurement. Serum samples were diluted 1+19 with ultrapure water after the addition of the 57Fe, 65Cu and 67Zn spikes. The proposed method was validated by the analysis of NIST SRM 1598 Inorganic Constituents in Bovine Serum, the levels found being in agreement with the certified values. Finally, the method was applied to the analysis of 21 serum samples from healthy blood donors.

References

  1. Biochemistry of the Essential Ultratrace Elements, ed. E. Frieden, Plenum Press, New York, 1984, p. 6 Search PubMed.
  2. K. G. Heumann, Int. J. Mass. Spectrom. Ion Processes, 1992, 118/119, 575 CrossRef.
  3. IUPAC, Pure Appl. Chem., 1995, 67, 1943 Search PubMed.
  4. R. M. Barnes, Fresenius' J. Anal. Chem., 1996, 355, 433 CAS.
  5. H. M. Crews, V. Ducros, J. Eagles, F. A. Mellon, P. Kastenmayer, J. B. Luten and B. A. McGaw, Analyst, 1994, 119, 2491 RSC.
  6. C. Veillon, K. Y. Patterson and P. B. Moser-Veillon, J. Anal. At. Spectrom., 1996, 11, 727 RSC.
  7. K. Y. Patterson, C. Veillon, P. B. Moser-Veillon and G. F. Wallace, Anal. Chim. Acta, 1992, 258, 317 CrossRef CAS.
  8. T. D. B. Lyon, S. Fletcher, G. S. Fell and M. Patriarca, Microchem. J., 1996, 54, 236 CrossRef CAS.
  9. P. G. Whittaker, T. Lind, J. G. Williams and A. L. Gray, Analyst., 1989, 114, 675 RSC.
  10. L. Moens, P. Verrept, R. Dams, U. Greb, G. Jung and B. Laser, J. Anal. At. Spectrom., 1994, 9, 1075 RSC.
  11. J. Riondato, F. Vanhaecke, L. Moens and R. Dams, J. Anal. At. Spectrom., 1997, 12, 933 RSC.
  12. C. Sariego Muñiz, J. M. Marchante Gayón, J. I. García Alonso and A. Sanz-Medel, J. Anal. At. Spectrom., 1999, 14, 193 RSC.
  13. F. Vanhaecke, L. Moens, R. Dams and P. Taylor, Anal. Chem., 1996, 68, 567 CrossRef CAS.
  14. F. Vanhaecke, L. Moens, R. Dams, I. Papadakis and P. Taylor, Anal. Chem., 1997, 69, 268 CrossRef CAS.
  15. D. Stuewer and N. Jakubowski, J. Mass Spectrom., 1998, 33, 579 CrossRef CAS.
  16. G. De Wannemacker, J. Riondato, F. Vanhaecke, L. Moens and R. Dams, presented at the 1999 European Winter Conference on Plasma Spectrometry, Pau, France, 1999.
  17. A. A. van Heuzen, T. Hoekstra and B. van Wingerden, J. Anal. At. Spectrom., 1989, 4, 483 RSC.
  18. H. P. Longerich, At. Spectrosc., 1989, 10, 112 CAS.
  19. G. Price Russ, III and J. M. Bazan, Spectrochim. Acta, Part B, 1987, 42, 49 CrossRef.
  20. J. I. García Alonso, Anal. Chim. Acta, 1995, 312, 57 CrossRef.
  21. R. Cornelis, B. Heinzow, R. F. M. Herber, J. Molin Christensen, O. M. Poulsen, E. Sabbioni, D. M. Templeton, Y. Thomassen, M. Vahter and O. Vesterberg, J. Trace Elem. Med. Biol., 1996, 10, 103 Search PubMed.
  22. K. J. R. Rosman and P. D. P. Taylor, J. Anal. At. Spectrom., 1999, 14, 5N Search PubMed.
  23. E. H. Evans and J. J. Giglio, J. Anal. At. Spectrom., 1993, 8, 1 RSC.
  24. H. Vanhoe, J. Goossens, L. Moens and R. Dams, J. Anal. At. Spectrom., 1994, 9, 177 RSC.
  25. T. W. May and R. H. Wiedmeyer, At. Spectrosc., 1998, 19, 150.
  26. S. Stürup, M. Hansen and C. Mølgaard, J. Anal. At. Spectrom., 1997, 12, 919 RSC.
  27. C. Latkoczy, T. Prohaska, G. Stingeder and M. Teschler-Nicola, J. Anal. At. Spectrom., 1998, 13, 561 RSC.
  28. F. Vanhaecke, G. De Wannemacker, L. Moens, R. Dams, C. Latkoczy, T. Prohaska and G. Stingeder, J. Anal. At. Spectrom., 1998, 13, 567 RSC.
  29. M. Montes Bayón, J. I. García Alonso and A. Sanz-Medel, J. Anal. At. Spectrom., 1998, 13, 1027 RSC.
  30. S. Caroli, A. Alimonti, E. Coni, F. Petrucci, O. Senofonte and N. Violante, CRC Crit. Rev. Anal. Chem., 1994, 24, 363 Search PubMed.
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