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DOI: 10.1039/A808231F (Paper) Reference Section for: J. Anal. At. Spectrom., 1999, 14, 639-644

Speciation of metal-carbohydrate complexes in fruit and vegetable samples by size-exclusion HPLC-ICP-MS

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Joanna Szpunar, Patrice Pellerin, Alexei Makarov, Thierry Doco, Pascale Williams and Ryszard Łobiński

Abstract

Kinetically inert and thermodynamically stable metal complexes with polysaccharides were detected in aqueous leachates and enzymatic digests of apple and carrot samples by size-exclusion chromatography with parallel refractometric and ICP-MS detection. The method developed allowed detection in the water-soluble fraction and the identification of a high molar mass polysaccharide fraction (>50 kDa) containing Pb, Ba, Sr, Ce and B, whereas other metals (Zn, Cu, Mg) eluted as complexes with low molar mass non-carbohydrate compounds. The majority of the metal-carbohydrate complexes were located in the solid water-insoluble fraction of the analysed samples. An extraction procedure with a mixture of pectinolytic enzymes was developed to release these species into the aqueous phase. The metal-binding carbohydrate component was identified as the dimer of rhamnogalacturonan-II, a pectic polysaccharide present in plant cell walls. The unidentified residual metal species contained less than 5% of the metals present.

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