Protein film cryovoltammetry: demonstrations with a 7Fe ([3Fe–4S] + [4Fe–4S]) ferredoxin

Abstract

Low-temperature protein film voltammetry, demonstrated here by studies of Fe–S clusters in the 7Fe ferredoxin from Thermoplasma acidophilum, offers a novel way to investigate active site redox reactions, allowing resolution of complex transformations and transient states, and providing an immediate and sensitive gauge of solvent effects.

References

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19. Peak potentials were completely restored although peak areas were ca. 10% smaller than measured at the start of the experiment.