A sulfite biosensor fabricated using electrodeposited polytyramine: application to wine analysis

Abstract

The development of a sulfite biosensor based on the immobilization of sulfite oxidase (SOD) in electrodeposited polytyramine is described. Electropolymerization of polytyramine and simultaneous immobilization of SOD on platinized glassy carbon (GC/Pt) were performed in an aqueous solution of 0.1 M tyramine containing SOD. The sulfite biosensor constructed with five voltammetric sweep cycles showed a sensitive response to sulfite with a linear calibration curve in the concentration range 0.002–0.3 mM sulfite, slope 2.70 µA l mmol^–1, and detection limit 0.001 mM sulfite (S/N = 3). Deposited polytyramine enhanced the selectivity of the biosensor by eliminating direct oxidation of sulfite and other interfering agents at the electrode. The biosensor was applied for the determination of sulfite in various types of wine samples, and the results obtained are in agreement with those using an AOAC method.

References

1. D. H. Allen, Food Technol. Aust., 1985, 37, 506.
2. P. Bruno, M. Caselli, A. Difano and A. Traini, Anal. Chim. Acta, 1979, 104, 239.
3. J. Hlavay and G. G. Guilbault, Anal. Chim. Acta, 1994, 299, 91.
4. S. Sasaki, Y. Arikawa, M. Shimomura, K. Ikebukuro and I. Karube, Anal. Commun., 1997, 34, 299.
5. Y. L. Huang, J. M. Kim and R. D. Schmid, Anal. Chim. Acta, 1992, 266, 317.
6. R. Keil, R. Hampp and H. Ziegler, Anal. Chem., 1989, 61, 1755.
7. M. D. Luque de Castro and J. M. Fernandez-Romero, Anal. Chim. Acta, 1995, 311, 281.
8. H. O. Beutler, Food Chem., 1994, 15, 157.
9. P. Linares, M. D. Luque de Castro and M. Valcarcel, Anal. Chim.Acta, 1989, 225, 443.
10. X. Xie, Z. Sakhsher, A. A. Suleiman, G. G. Guilbault, Z. Yang and Z. Sun, Talanta, 1994, 41, 317.
11. D. A. Paulls and A. Townshend, Analyst, 1995, 120, 467.
12. F. Wu, Z. He, H. Meng and Y. Zeng, Analyst, 1998, 123, 2109.
13. N. Imaizumi, K. Hayakawa, N. Okubo and M. Miyazaki, Chem.Pharm. Bull., 1981, 29, 3755.
14. I. G. Casella and R. Marchese, Anal. Chim. Acta, 1995, 311, 199.
15. H. J. Kim and Y. K. Kim, J. Food Sci., 1986, 51, 1360.
16. M. B. Wygant, J. A. Statler and A. Henshall, J. AOAC Int., 1997, 80, 1374.
17. U. Korell and R. B. Lennox, J. Electroanal. Chem., 1993, 351, 137.
18. L. T. Ng, Y. T. Yuan and H. Zhao, Electroanalysis, 1998, 10, 1119.
19. J. Svitel, M. Stredansky, A. Pizzanello and S. Miertus, Electroanalysis, 1998, 10, 591.
20. M. O. Rezende and H. A. Motolla, Analyst, 1994, 119, 2093.
21. Official Methods of Analysis of the Association of Official Analytical Chemists, AOAC, Arlington, VA, 14th edn., 1984, pp. 391–392.
22. V. J. Smith, Anal. Chem., 1987, 59, 2256.
23. T. Fenong, Anal. Chim. Acta, 1986, 184, 287.
24. M. A. Nabirahmi and R. R. Vaid, Anal. Lett., 1991, 24, 551.
25. A. Mulchandani, C. A. Groom and J. H. T. Luong, J. Biotechnol., 1991, 18, 93.
26. M. Massom and A. Townshend, Anal. Chim. Acta, 1986, 179, 399.
27. T. Yao, M. Satomura and T. Nakahara, Talanta, 1994, 41, 2113.
28. S. B. Adeloju, S. J. Shaw and G. G. Wallace, Electroanalysis, 1994, 6, 865.
29. S. B. Adeloju, J. N. Barisci and G. G. Wallace, Anal. Chim. Acta, 1996, 332, 145.
30. M. Situmorang, D. B. Hibbert, J. J. Gooding and D. Barnett, in Proceedings of the Australian International Symposium on AnalyticalScience 1999, ed. P. Iles and E. Knox, Melbourne, Australia, 1999, p. 274.
31. J. E. Dubois, P. C. Lacaze and M. C. Pham, J. Electroanal. Chem., 1981, 117, 233.
32. M. C. Pham, P. C. Lacaze and J. E. Dubois, J. Electrochem. Soc., 1984, 131, 777.
33. M. Situmorang, J. J. Gooding, D. B. Hibbert and D. Barnett, Biosens. Bioelectron., 1998, 13, 953.
34. M. Situmorang, J. J. Gooding and D. B. Hibbert, Anal. Chim. Acta, 1999, 394, 211.
35. M. Situmorang, D. B. Hibbert and J. J. Gooding, Electroanalysis, in the press.
36. D. Barnett, Food Technol. Aust., 1985, 37, 503.
37. R. B. Boulton, V. L. Singleton, L. F. Bisson and R. E. Kunkee, Principles and Practices of Winemaking, Chapman and Hall, NewYork, 1996, p. 84.
38. B. W. Zoecklein, K. C. Fugelsang, B. H. Gump and F. S. Nury, Wine Analysis and Production, Chapman and Hall, New York, 1995, p. 178.