Solid-phase microextraction for the determination of pethidine and methadone in human urine using gas chromatography with nitrogen–phosphorus detection

Abstract

A simple and rapid analytical method is presented for the determination of pethidine (meperidine) and methadone in human urine using solid-phase microextraction (SPME) and gas chromatography with nitrogen–phosphorus detection (GC-NPD). After the analytes had been partitioned between an extracting phase and the aqueous sample matrix, the needle of the coating fiber assembly was injected directly into the GC injector. The analytes were thermally desorbed in the heated injector (240 °C) and subsequently separated and detected by the GC-NPD system. The factors influencing the SPME method, such as the salt (NaCl) effect (15%), pH (pH 11), and equilibration time (30 min), were optimized. The calibration graphs for urine samples showed a good linearity. The detection limit was below 1 ng ml^–1 for both drugs.

References

1. A. G. GilmanL. S. GoodmanThe Pharmacological Basis of Therapeutics, Macmillan, New York, 7th edn. 1985pp. 513–519.
2. IOC Medical Commission : Medical Code and Explanatory Document, 1996, ch. II.
3. N. Chikhi-Chorfi, C. Pham-Huy, H. Galons, N. Manuel, W. Lowenstein, J.-M. Warnet and J.-R. Claude, J. Chromatogr., B: Biomed. Appl., 1998, 718, 278.
4. J. F. Furness, A. Weekes, M. Clench, K. Wolff, I. Barnes, W. M. Alistair and M. Cooke, J. Liq. Chromatogr., 1994, 17, 4431.
5. A. M. Bermejo, C. Remuinan, P. Fernandez and M. J. Tabernero, Anal. Lett., 1998, 31, 645.
6. M. J. Bogusz, R.-D. Maier, K.-D. Kruger and U. Kohls, J. Anal. Toxicol., 1998, 22, 549.
7. M. E. Alburges, W. Huang, R. L. Foltz and D. E. Moody, J. Anal. Toxicol., 1996, 20, 362.
8. S. Rudaz and J.-L. Veuthey, J. Pharm. Biomed. Anal., 1996, 14, 1271.
9. C. L. Arthur and J. B. Pawliszyn, Anal. Chem., 1990, 62, 2145.
10. C. L. Arthur, L. M. Killam, K. D. Buchholz and J. B. Pawliszyn, Anal. Chem., 1992, 64, 1960.
11. S.-W. Myung, H.-K. Min, S. Kim, M. Kim, J.-B. Cho and T.-J. Kim, J. Chromatogr., B: Biomed. Appl., 1998, 716, 359.
12. S.-W. Myung, M. Kim, H.-K. Min, E.-A. Yoo and K.-R. Kim, J. Chromatogr., B: Biomed. Appl., 1998, 727, 1.
13. X.-P. Lee, T. Kumazawa, K. Sato and O. Suzuki, J. Chromatogr. Sci., 1997, 35, 302.
14. T. J. Clark and J. E. Bunch, J. Chromatogr. Sci., 1997, 35, 209.
15. S. Magic, A. B. Boland, K. Jinno and J. B. Pawliszyn, J. Chromatogr., 1996, 736, 219.
16. J. Pawliszyn, Solid Phase Microextraction: Theory and Practice, Wiley–VCH, New York, 1997, pp. 130–131.
17. A. C. Moffat, Clarke's Isolation and Identification of Drugs, Pharmaceutical Press, London, 1986.
18. M. Chiarotti and R. Marsili, J. Microcol . Sep., 1994, 6, 577.