Determination of malondialdehyde in traditional fish products by HPLC

(Note: The full text of this document is currently only available in the PDF Version )

John Tsaknis, Stavros Lalas and Evangelos Evmorfopoulos

The oxidation state of traditional fish products was measured by determining the malondialdehyde (MDA) level by HPLC and the results were compared to those given by a spectrophotometric method. The procedure involves oxidation of the products by incubation at 40 °C for 3 d. Samples were steam distilled in a Kjeldahl distillation apparatus and the MDA was determined in the aqueous distillates by HPLC, using a µ-Bondapak C18 column, with mixed mobile phase of 1% acetic acid–acetonitrile (85 + 15; v/v). A total time of 2 min was necessary to assay each distillate and only MDA was detected. MDA can be determined at a level of 1.5 × 10–8 mol l–1. The highest rate of oxidation of the samples, as shown by the changes in the TBA test and MDA concentration determined by HPLC, was observed in smoked fish and the lowest in dried–salted fish.


  1. A. Aitken, I. M. Mackie, J. H. Merritt. and M. L. Windsor, Fish Handling and Processing, HM Stationery Office, Edinburgh, 2nd ed., 1982 Search PubMed.
  2. W. W. Nawar, in Food Chemistry, ed. O. R. Fennema, Marcel Dekker, New York, 1985, pp. 140–213 Search PubMed.
  3. H. H. Draper and M. Hadley, Methods Enzymol., 1990, 186, 421 CAS.
  4. L. K. Dahle, G. Hill and R. T. Holman, Arch. Biochem. Biophys., 1962, 98, 253 CrossRef CAS.
  5. E. Kishida, M. Oribe, K. Mochizuki and S. Kojo, Biochim. Biophys. Acta, 1990, 1045, 187 CrossRef CAS.
  6. H. Ohkawa, N. Ohishi and K. Yagi, J. Lipid Res., 1978, 19, 1053 Search PubMed.
  7. Y. Kakuda, D. W. Stanley and F. R. van de Voort, J. Am. Oil. Chem. Soc., 1981, 58, 773 CAS.
  8. T. Hirayama, J. Assoc. Off. Anal. Chem., 1983, 66, 304 Search PubMed.
  9. J. Tsaknis, S. Lalas, M. Hole, G. Smith and V. Tychopoulos, Analyst, 1998, 123, 325 RSC.
  10. B. G. Tarladgis, B. M. Watts, M. T. Younathan and L. R. Dugan, Jr., J. Am. Oil. Chem. Soc., 1960, 37, 44 CAS.
  11. R. C. Sans, I. V. Romero and M. G. Chozas, Fat. Sci. Technol., 1993, 95, 225 Search PubMed.
  12. S. Zlatanos and A. N. Sagrados, Fat. Sci. Technol., 1993, 95, 66 Search PubMed.
  13. U. Kazuaki, T. Morishita. and T. Takahashi, Nippon. Suisan. Gakkaishi., 1987, 53, 1609 Search PubMed.
  14. A. Viga and O. Grahl-Nielsen, Comp. Biochem. Physiol., 1990, 96B, 721 Search PubMed.
  15. M. C. Erickson, Comp. Biochem. Physiol., 1992, 101, 171 Search PubMed.
  16. B. G. Tarladgis, A. M. Pearson and L. R. Dugan, Jr., J. Am. Oil. Chem. Soc., 1962, 39, 34 CAS.