DOI: 10.1039/A900922A
(Paper)
Reference Section for: Analyst, 1999, 124, 801-804
Fully automated determination of vitamins A and E in milk-based products using a robotic station–HPLC arrangement
(Note: The full text of this document is currently only available in the PDF Version )
L. Gámiz-Gracia, A. Velasco-Arjona and M. D. Luque de Castro
Abstract
An automated method, which includes an extraction procedure based on the AOAC official method of analysis for vitamins A and E in milk-based infant formula, was developed. The whole procedure is carried out automatically in a robotic station coupled to a liquid chromatograph with a UV detector for individual separation and quantification of the vitamins. Different commercial products (milk, infant formula and nutritional supplements) were analysed, and the data were compared with those obtained using the manual AOAC extraction method. Relative standard deviations using the automated method were 5.92 and 2.57% for vitamin A and E, respectively.
References
- A. Salvador, M. A. Jaime, M. de la Guardia and G. Becerra, Anal. Commun., 1998, 35, 53 RSC.
- M. M. Delgado Zamarreno, A. Sánchez Pérez, C. Gómez Pérez and J. Hernández Méndez, J. Chromatogr., 1992, 623, 69 CrossRef.
- B. Y. Gong and J. W. Ho, J. Liq. Chromatogr. Relat. Technol., 1997, 20, 2389 CAS.
- P. C. H. Hollman, J. H. Slangen, P. J. Wagstaffe, U. Faure, D. A. T. Southgate and P. M. Finglas, Analyst, 1993, 118, 475 RSC.
- D. M. Sullivan and D. E. Carpenter, in Methods of Analysis for Nutrition Labeling, AOAC International, Arlington, VA, 1993, pp. 557 and 591(methods 992.06 and 992.03 for viamins A and E, respectively) Search PubMed.
- A. Velasco-Arjona, J. J. Manclús, A. Montoya and M. D. Luque de Castro, Talanta, 1997, 45, 371 CrossRef CAS.
- A. Velasco-Arjona and M. D. Luque de Castro, Anal. Chim. Acta, 1996, 333, 205 CrossRef CAS.
- A. Velasco-Arjona, J. A. García-Garrido, R. Quiles-Zafra and M. D. Luque de Castro, Talanta, 1998, 46, 969 CrossRef CAS.
- A. Velasco-Arjona, A. Izquierdo and M. D. Luque de Castro, Analyst, 1998, 123, 509 RSC.
- A. Velasco-Arjona and M. D. Luque de Castro, Analyst, 1997, 122, 123 RSC.
- K. McCambly, R. C. Kelly, T. Johnson, J. E. Johnson and W. C. Brown, J. Anal. Toxicol., 1997, 21, 438 CAS.
- L. Cuadros Rodríguez, A. M. García Campaña, C. Jiménez Linares and M. Román Ceba, Anal. Lett., 1993, 26, 1243.
- A. M. García Campaña, L. Cuadros Rodríguez, F. Alés Barrero, M. Román Ceba and J. L. Sierra Fernández, Trends Anal. Chem., 1997, 16, 381 CrossRef CAS.