Highly sensitive spectrofluorimetric determination of hydrogen peroxide with β-cyclodextrin–hemin as catalyst

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Zhihong Liu, Ruxiu Cai, Luyuan Mao, Houping Huang and Wanhong Ma


Abstract

β-Cyclodextrin (CD)–hemin was studied as a replacement for horseradish peroxidase (HRP) in the spectrofluorimetric determination of H2O2. The optimum concentrations of substrate and β-CD–hemin were studied. pH 10.4 was found to be the optimum value for the experiment. The influence of different buffers on the peroxidase activity of β-CD–hemin was tested and it was found that the peroxidase activity of β-CD-hemin was enhanced by NH3. The oxidation product 2,2′-dihydroxy-4,4′-dimethylbiphenyl was monitored fluorimetrically, with a detection limit of 3.4 × 10–9 mol l–1 of H2O2. A linear calibration graph was obtained over the H2O2 concentration range 3 × 10–8–8 × 10–6 mol l–1, with a correlation coefficient of 0.9993. The relative standard deviation at an H2O2 concentration of 2 × 10–6 mol l–1 was 1.8%.


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