Sol–gel horseradish peroxidase biosensor for the chemiluminescent flow determination of hydrogen peroxide

Abstract

A chemiluminescent (CL) H₂O₂ sensor based on horseradish peroxidase (HRP) immobilized by the sol–gel method has been proposed in this paper. A new process of fabricating a non-cracking HRP encapsulated sol–gel membrane has been suggested. Flow injection analysis (FIA) was used to give reproducible results. Under optimum conditions, the sensor showed a linear response toward hydrogen peroxide in the range 0.01–2 mM with a detection limit of 8 µM. The linear relative coefficient r = 0.998. The sensor showed rapid response, good reproducibility (relative standard deviation = 2.4%) and a long lifetime of at least two months. The proposed sensor was used to determine a 30% H₂O₂ solution that has been stored for a long time. The results were in good agreement with the standard volumetric method.

References

1. K. M. Wang, Theories and Methods of Optical Chemical Sensor, Hunan Education Press, Changsha, 1994, ch. 8.
2. U. Parang, P. N. Prasad, F. V. Bright, K. Ramanathan, N. D. Kumar, B. D. Malhotra, M. N. Kamalasanan and S. Chandra, Anal. Chem., 1994, 66, 3139.
3. J. Li, S. N. Tan and H. Ge, Anal. Chim. Acta, 1996, 335, 137.
4. J. Wang, P. V. A. Pamidi and D. S. Park, Anal. Chem., 1996, 68, 2705.
5. J. W. Aylott, D. J. Richardson and D. A. Russell, Analyst, 1997, 122, 77.
6. D. J. Blyth, J. W. Aylott, D. J. Richardson and D. A. Russell, Analyst, 1995, 120, 2750.
7. O. Lev, M. Tsionsky, L. Rabinovich, V. Glezer, S. Sampath, I. Pankratov and J. Gun, Anal. Chem., 1995, 67, 22A.
8. B. C. Dave, B. Dunn, J. S. Valentine and J. I. Zink, Anal. Chem., 1994, 66, 1120A.
9. J. Lin and C. W. Brown, Trends Anal. Chem., 1997, 16, 200.
10. S. C. Kraus, R. Czolk, J. Reichert and H. J. Ache, Sens. Actuators B, 1993, 15–16, 199.
11. R. W. Marshall and T. D. Gibbson, Anal. Chim. Acta, 1992, 266, 309.
12. F. Preuschoff, U. Spohn, G. Blankenstein, K. H. Mohr and M. R. Kula, Fresenius' J. Anal. Chem., 1993, 346, 924.
13. A. Berger and L. J. Blum, Enzyme Microb. Technol., 1994, 16, 979.
14. Y. X. Zhou and G. Y. Zhu, Chin. J. Anal. Chem., 1997, 25, 222.
15. A. N. Diaz, M. C. R. Peinado and M. C. T. Minguez, Anal. Chim. Acta, 1998, 363, 221.
16. J. Z. Li, Z. Z. Zhang and L. Li, Talanta, 1994, 41, 1999.
17. E. C. Horning, Organic Synthesis collective Vol. III (20–29), John Wiley, 1935; Chinese translation by Organ. Chem. Group of Nanjing University, Science Press, Beijing, 1981, pp. 42–43.
18. K. M. Wang, J. Li, X. H. Yang, F. L. Shen and X. Wang, Technical Digest of the Seventh International Meeting on Chemical Sensors, Beijing, 1998, p. 586.