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DOI: 10.1039/A809656B (Paper) Reference Section for: Anal. Commun., 1999, 36, 47-50

A water-soluble tetrazolium salt useful for colorimetric cell viability assay

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Hideyuki Tominaga, Munetaka Ishiyama, Fumio Ohseto, Kazumi Sasamoto, Tomoyuki Hamamoto, Keiji Suzuki and Masami Watanabe

Abstract

The application of a tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt (WST-8), to cell viability assays and in vitro drug sensitivity tests is described. With a higher sensitivity as a chromogenic indicator for cell viability compared with conventional tetrazolium salts, WST-8 produced results of cell viability and IC50 values that were in good agreement, respectively, with the MTT method and [3H]thymidine uptake method.

References

  1. J. M. Webb, J. B. Brouwer and E. A. Brouwer, Toxicol. Appl. Pharmacol., 1967, 10, 300 CAS.
  2. E. Borenfreund and J. A. Puerner, Toxicol. Lett., 1985, 24, 119 CrossRef CAS.
  3. K. Saotome, H. Morita and M. Umeda, Toxic. In Vitro, 1989, 3, 317 Search PubMed.
  4. T. Mosmann, J. Immunol. Methods, 1983, 65, 55 CrossRef CAS.
  5. M. Ishiyama, M. Shiga, K. Sasamoto and M. Mizoguchi, Chem. Pharm. Bull., 1993, 41, 1118 CAS.
  6. P. Heiss, S. Bernatz, G. Bruchelt and R. Senekowitsch-Schmidtke, Anticancer Res., 1997, 17, 3177 Search PubMed.
  7. M. Fujiwara, N. Nagano, K. Monden, M. Misumi, K. Kageyama, K. Yamamoto and N. Miwa, Free Rad. Res., 1997, 27, 97 Search PubMed.
  8. A. M. Gressner, B. Lahme, H.-G. Mannherz and B. Polzar, J. Hepatol., 1997, 26, 1079 CrossRef CAS.
  9. A. M. Gressner, B. Lahme and S. Roth, Biochem. Biophys. Res. Commun., 1997, 231, 457 CrossRef CAS.
  10. E. Nissen, D. Vollenbroich and G. Pauli, In Vitro Cell Dev. Biol.—Animal, 1997, 33, 28 Search PubMed.
  11. M. Ishiyama, H. Tominaga, M. Shiga, K. Sasamoto, Y. Ohkura and K. Ueno, Biol. Pharm. Bull., 1996, 19, 1518 Search PubMed.
  12. M. Kawanishi, J. Virology, 1997, 71, 3319 Search PubMed.
  13. S. Wagner, W. Beil, J. Westermann, R. P. H. Logan, C. T. Bock, C. Trautwein, J. S. Bleck and M. P. Manns, Gastroenterology, 1997, 113, 1836 Search PubMed.
  14. O. P. Mgbonyebi, J. Russo and I. H. Russo, Int. J. Oncology, 1998, 12, 865 Search PubMed.
  15. K. Hamasaki, K. Kogure and K. Ohwada, Toxicon, 1996, 34, 490 CrossRef CAS.
  16. T. Kawase, S. Ogata, M. Orikasa and D. M. Burns, Calcif. Tissue Int., 1995, 57, 359 CAS.
  17. T. Takenouchi and E. Munekata, Life Sci., 1995, 56, PL479 CrossRef CAS.
  18. M. Ishiyama, Y. Miyazono, K. Sasamoto, Y. Ohkura and K. Ueno, Talanta, 1997, 44, 1299 CrossRef CAS.
  19. D. A. Scudiero, R. H. Shoemaker, K. D. Paull, A. Monks, S. Tierney, T. H. Nofziger, M. J. Currens, D. Seniff and M. R. Boyd, Cancer Res., 1988, 48, 4827 CAS.
  20. A. H. Cory, T. C. Owen, J. A. Barltrop and J. G. Cory, Cancer Commun., 1991, 3, 207 Search PubMed.
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