View PDF Version
 
DOI: 10.1039/A809656B (Paper) Reference Section for: Anal. Commun., 1999, 36, 47-50

A water-soluble tetrazolium salt useful for colorimetric cell viability assay

(Note: The full text of this document is currently only available in the PDF Version )

Hideyuki Tominaga, Munetaka Ishiyama, Fumio Ohseto, Kazumi Sasamoto, Tomoyuki Hamamoto, Keiji Suzuki and Masami Watanabe

Abstract

The application of a tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt (WST-8), to cell viability assays and in vitro drug sensitivity tests is described. With a higher sensitivity as a chromogenic indicator for cell viability compared with conventional tetrazolium salts, WST-8 produced results of cell viability and IC50 values that were in good agreement, respectively, with the MTT method and [3H]thymidine uptake method.

References

  1. J. M. Webb, J. B. Brouwer and E. A. Brouwer, Toxicol. Appl. Pharmacol., 1967, 10, 300 CAS .
  2. E. Borenfreund and J. A. Puerner, Toxicol. Lett., 1985, 24, 119 CrossRef CAS .
  3. K. Saotome, H. Morita and M. Umeda, Toxic. In Vitro, 1989, 3, 317 Search PubMed .
  4. T. Mosmann, J. Immunol. Methods, 1983, 65, 55 CrossRef CAS .
  5. M. Ishiyama, M. Shiga, K. Sasamoto and M. Mizoguchi, Chem. Pharm. Bull., 1993, 41, 1118 CAS .
  6. P. Heiss, S. Bernatz, G. Bruchelt and R. Senekowitsch-Schmidtke, Anticancer Res., 1997, 17, 3177 Search PubMed .
  7. M. Fujiwara, N. Nagano, K. Monden, M. Misumi, K. Kageyama, K. Yamamoto and N. Miwa, Free Rad. Res., 1997, 27, 97 Search PubMed .
  8. A. M. Gressner, B. Lahme, H.-G. Mannherz and B. Polzar, J. Hepatol., 1997, 26, 1079 CrossRef CAS .
  9. A. M. Gressner, B. Lahme and S. Roth, Biochem. Biophys. Res. Commun., 1997, 231, 457 CrossRef CAS .
  10. E. Nissen, D. Vollenbroich and G. Pauli, In Vitro Cell Dev. Biol.—Animal, 1997, 33, 28 Search PubMed .
  11. M. Ishiyama, H. Tominaga, M. Shiga, K. Sasamoto, Y. Ohkura and K. Ueno, Biol. Pharm. Bull., 1996, 19, 1518 Search PubMed .
  12. M. Kawanishi, J. Virology, 1997, 71, 3319 Search PubMed .
  13. S. Wagner, W. Beil, J. Westermann, R. P. H. Logan, C. T. Bock, C. Trautwein, J. S. Bleck and M. P. Manns, Gastroenterology, 1997, 113, 1836 Search PubMed .
  14. O. P. Mgbonyebi, J. Russo and I. H. Russo, Int. J. Oncology, 1998, 12, 865 Search PubMed .
  15. K. Hamasaki, K. Kogure and K. Ohwada, Toxicon, 1996, 34, 490 CrossRef CAS .
  16. T. Kawase, S. Ogata, M. Orikasa and D. M. Burns, Calcif. Tissue Int., 1995, 57, 359 CAS .
  17. T. Takenouchi and E. Munekata, Life Sci., 1995, 56, PL479 CrossRef CAS .
  18. M. Ishiyama, Y. Miyazono, K. Sasamoto, Y. Ohkura and K. Ueno, Talanta, 1997, 44, 1299 CrossRef CAS .
  19. D. A. Scudiero, R. H. Shoemaker, K. D. Paull, A. Monks, S. Tierney, T. H. Nofziger, M. J. Currens, D. Seniff and M. R. Boyd, Cancer Res., 1988, 48, 4827 CAS .
  20. A. H. Cory, T. C. Owen, J. A. Barltrop and J. G. Cory, Cancer Commun., 1991, 3, 207 Search PubMed .
Click here to see how this site uses Cookies. View our privacy policy here.