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DOI: 10.1039/A809150A (Paper) Reference Section for: Anal. Commun., 1999, 36, 5-7

Preliminary study on fluorimetric detection of aflatoxins Q1, P1 and B1 using heptakis-di-O-methyl-β-cyclodextrin as post-column HPLC reagent

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B. I. Vázquez, C. A. Fente, C. M. Franco, A. Cepeda, G. Mahuzier and P. Prognon

Abstract

Post-column fluorimetric detection for the determination of aflatoxins Q1, P1 and B1 was carried out by using HPLC with a 2.0 mm id column. The post-column reagent consisted of a 10–2M aqueous solution of heptakis-di-O-methyl-β-cyclodextrin. The fluorescence enhancement achieved was 37- and 27-fold for AFQ1 and AFB1, respectively, whereas the AFP1 signal was increased just about 2-fold. With the proposed method, aflatoxins Q1, P1 and B1 can be simultaneously determined in human urine. The detection limits (S/N = 3) were as follows: 0.7 ng ml–1 for AFQ1, 0.5 ng ml–1 for AFP1 and 0.3 ng ml–1 for AFB1.

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