Enzymatic Preparation of Sphingosine 1-Phosphate

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Eiji Morigaki, Yoshie Miura, Kyoya Takahata, Mikiro Tada, Shuhei Nakajima and Naomichi Baba


Abstract

Sphingosine 1-phosphate was prepared from sphingosine by two steps involving conversion of sphingosine to phosphatidylsphingosine catalyzed by phospholipase D and enzymatic hydrolysis of the intermediate to a diacyglycerol and sphingosine 1-phosphate with phospholipase C.


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