Eiji Morigaki, Yoshie Miura, Kyoya Takahata, Mikiro Tada, Shuhei Nakajima and Naomichi Baba
Sphingosine 1-phosphate was prepared from sphingosine by two steps involving conversion of sphingosine to phosphatidylsphingosine catalyzed by phospholipase D and enzymatic hydrolysis of the intermediate to a diacyglycerol and sphingosine 1-phosphate with phospholipase C.