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DOI: 10.1039/A803038C (Paper) Reference Section for: J. Anal. At. Spectrom., 1998, 13, 749-754

Speciation analysis for biomolecular complexes of lead in wine by size-exclusion high-performance liquid chromatography-inductively coupled plasma mass spectrometry

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Joanna Szpunar, Patrice Pellerin, Alexei Makarov, Thierry Doco, Pascale Williams, Bernard Medina and Ryszard Łobiński

Abstract

Size-exclusion chromatography with ICP-MS detection was developed for speciation of lead in wine. The method required minimal sample preparation and allowed routine analysis of wine samples, showing the distribution of lead among biomolecular compounds within 30 min. The quantification of lead bound to biomolecules was done by comparing the chromatographic peak area with that of a signal obtained by flow-injection ICP-MS analysis. A study carried out on a population of 20 wines of different origin showed that mineral lead on which the toxicity conclusions were based may not exist in wine. Lead was found to be associated with one major biomolecular (ca. 10 kDa) species present in all wine samples, and one to three minor compounds whose number depended on the wine sample. The dominant species which accounted for 40–95% of lead was identified as the complex formed with the dimer of a pectic polysaccharide, rhamnogalacturonan II. Other minor species with apparent molecular masses in the range 500–3000 Da were not identified.

References

  1. V. Legrand, B. Médina, J. P. Grenon and M. Pauchet, Analusis, 1991, 19, M39 CAS .
  2. G. C. Elinder, B. Lind, B. Nilsson and A. Oskarsson, Food Addit. Contam., 1988, 5, 641 .
  3. P. L. Teissedre, M. T. Cabanis, F. Champagnol and J. C. Cabanis, Am. J. Enol. Vitic., 1994, 45, 220 Search PubMed .
  4. K. Cezard–Hagenord, Toxicologie chez l'Homme, Lavoisier, Tec-Doc, Paris, 1992 Search PubMed .
  5. International Methods of Wine Analysis, Annexe C (Maximum Levels of Different Elements in Wine), Paris, France, 1990 Search PubMed .
  6. L. Jorhem and B. Sundstrom, At. Spectrosc., 1995, 16, 226 CAS .
  7. W. R. Mindak, J. Assoc. Off. Anal. Chem., 1994, 77, 1023 Search PubMed .
  8. M. R. Matthews and P. J. Parsons, At. Spectrosc., 1993, 14, 41 CAS .
  9. H. Eschnauer, L. Jakob, H. Meierer and R. Neeb, Mikrochim. Acta, 1989, III, 291 .
  10. J. Goossens, T. De Smaele, L. Moens and R. Dams, Fresenius' J. Anal. Chem., 1993, 347, 119 CrossRef CAS .
  11. J. R. Dean, L. Ebdon and R. C. Massey, Food Addit. Contam., 1990, 7, 109 CAS .
  12. S. Augagneur, B. Medina and F. E. Grousset, Fresenius' J. Anal. Chem., 1997, 357, 1149 CrossRef CAS .
  13. R. Łobiński, P. L. Teissedre, J. Szpunar, J. C. Cabanis and F. C. Adams, J. Assoc. Off. Anal. Chem., 1993, 76, 1262 Search PubMed .
  14. R. Łobiński, C. Witte, F. C. Adams, P. L. Teissedre, J. C. Cabanis and C. F. Boutron, Nature, 1994, 370, 24 CrossRef CAS .
  15. P. L. Teissedre, R. Łobiński, M. T. Cabanis, J. Szpunar, J. C. Cabanis and F. C. Adams, Sci. Total Environ., 1994, 153, 247 CAS .
  16. C. Marin and P. Ostapczuk, Fresenius' J. Anal. Chem., 1992, 343, 881 CAS .
  17. G. R. Scollary, Analusis, 1997, 25, M26 CAS .
  18. T. Arcos, M. C. Ancin, J. C. Echeverria, A. Gonzalez and J. J. Garrido, J. Agric. Food Chem., 1993, 41, 2333 CrossRef CAS .
  19. M. A. Baldo, C. Bragato and S. Daniele, Analyst, 1997, 122, 1 RSC .
  20. A. M. Green, A. C. Clarc and G. R. Scollary, Fresenius' J. Anal. Chem., 1997, 358, 711 CrossRef CAS .
  21. S. Minguez, P. Hernandez and M. Gonzalo, in Oenologie 95, ed. Lonvaud-Funel, A., Lavoisier, Paris, 1996, pp. 632–635 Search PubMed .
  22. H. R. Eschnauer and G. Scollary, Vitic. Enol. Sci., 1996, 51, 6 Search PubMed .
  23. T. Doco and J. M. Brillouet, Carbohydr. Res., 1993, 243, 333 CrossRef CAS .
  24. P. Pellerin, T. Doco, S. Vidal, P. Williams, J. M. Brillouet and M. A. O'Neill, Carbohydr. Res., 1996, 290, 183 CrossRef CAS .
  25. M. A. O'Neill, D. Warrenfeltz, K. Kates, P. Pellerin, T. Doco, A. G. Darvill and P. Albersheim, J. Biol. Chem., 1996, 271, 22923 CrossRef CAS .
  26. T. Ishii and T. Matsunaga, Carbohydr. Res., 1996, 284, 1 CrossRef CAS .
  27. M. Kobayashi, T. Matoh and J. L. Azuma, Plant Physiol., 1996, 110, 1017 CAS .
  28. P. Pellerin, M. A. O'Neill, C. Pierre, M. T. Cabanis, A. G. Darvill, P. Albersheim and M. Moutounet, J. Int. Sci. Vigne Vin, 1997, 31, 33 Search PubMed .
  29. T. Doco, N. Quellec, M. Moutounet and P. Pellerin, Am. J. Enol. Vitic., in the press Search PubMed .
  30. K. Sutton, R. M. C. Sutton and J. A. Caruso, J. Chromatogr. A, 1997, 789, 85 CrossRef CAS .
  31. T. Matsunaga, T. Ishiii and H. Watanabe, Anal. Sci., 1996, 12, 673 CrossRef CAS .
  32. S. Augagneur, B. Médina, J. Szpunar and R. Łobiński, J. Anal. At. Spectrom., 1996, 11, 713 RSC .
  33. A. Itoh, C. Kimata, H. Miwa, H. Sawatari and H. Haraguchi, Bull. Chem. Soc. Jpn., 1996, 69, 3469 CAS .
  34. P. Pellerin and J. C. Cabanis, In Elements d'Œnologie, ed. Flanzy, C., Lavoisier, Paris, 1998, p. 39, in the press Search PubMed .
  35. P. Hocquellet, Analusis, 1997, 25, 25 CAS .
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