Ultraviolet absorbance and inductively coupled plasma mass spectrometric detection for capillary electrophoresis—A comparison of detection modes and interface designs

(Note: The full text of this document is currently only available in the PDF Version )

Karen L. Sutton, Clayton B’hymer and Joseph A. Caruso


Abstract

A comparison of ICP-MS and UV absorbance as detectors for capillary electrophoresis (CE) was performed. A high efficiency nebulizer (HEN) with cyclone spray chamber was found to yield detection limits in the low parts-per-billion range while UV detection gave detection limits in the parts-per-million range for the same separation of the lanthanides. Electrokinetic and hydrostatic methods of injection were employed, and it was found that both methods gave similar detection limits and precision values for the separation, for both methods of detection. The use of a traditional concentric nebulizer as part of the CE–ICP-MS interface was evaluated and compared with the HEN. The optimum make-up buffer liquid flow rate was evaluated for the concentric nebulizer and detection limits for the lanthanide separation were calculated. Detection limits with the concentric nebulizer were not improved compared with UV detection, illustrating that the choice of nebulizer for the interface is of primary importance for improved analytical sensitivity. Attempts to use an oscillating capillary nebulizer as part of a CE–ICP-MS interface are also described.


References

  1. A. G. Ewing, R. A. Wallingford and T. M. Olefirowicz, Anal. Chem., 1989, 61, 292A CAS.
  2. W. G. Kuhr, Anal. Chem., 1990, 62, 403R CAS.
  3. P. Jandik, W. R. Jones, A. Weston and P. R. Brown, LC-GC, 1991, 9, 634 Search PubMed.
  4. M. Chen and R. M. Cassidy, J. Chromatogr., 1992, 602, 227 CrossRef CAS.
  5. Y. Shi and J. S. Fritz, J. Chromatogr., 1993, 640, 473 CrossRef CAS.
  6. T. Hirokawa, N. Aoki and Y. Kiso, J. Chromatogr., 1984, 312, 11 CrossRef CAS.
  7. F. Foret, S. Fanali, L. Ossicini and P. Bocek, J. Chromatogr., 1989, 470, 299 CrossRef CAS.
  8. L. Lin, J. Wang and J. Caruso, J. Chromatogr. Sci., 1995, 33, 177 CAS.
  9. J. J. Corr and J. F. Anacleto, Anal. Chem., 1996, 68, 2155 CrossRef CAS.
  10. J. Olivares, N. T. Ngupta, C. R. Yonker and R. D. Smith, Anal. Chem., 1987, 59, 1230 CrossRef CAS.
  11. K. L. Sutton, R. M. C. Sutton and J. A. Caruso, J. Chromatogr., 1997, 789, 85 CrossRef CAS.
  12. S. Röllin, Z. Kopatjtic, B. Wernli and B. Magyar, J. Chromatogr., 1996, 739, 139 CrossRef.
  13. K. Kawabata, Y. Kishi, O. Kawaguchi, Y. Watanabe and Y. Inoue, Anal. Chem., 1991, 63, 2137 CrossRef CAS.
  14. J. W. Olesik, J. A. Kinzer and S. V. Olesik, Anal. Chem., 1995, 67, 1 CrossRef CAS.
  15. Y. Liu, V. Lopez-Avila, J. J. Zhu, D. R. Weiderin and W. F. Beckert, Anal. Chem., 1995, 67, 2020 CrossRef CAS.
  16. Q. Lu, S. M. Bird and R. M. Barnes, Anal. Chem., 1995, 67, 2949 CrossRef CAS.
  17. Q. Lu and R. M. Barnes, Microchem. J., 1996, 54, 129 CrossRef CAS.
  18. J. A. Kinzer, J. W. Olesik and S. V. Olesik, Anal. Chem., 1996, 68, 3250 CrossRef CAS.
  19. M. L. Magnuson, J. T. Creed and C. A. Brockhoff, J. Anal. At. Spectrom., 1997, 12, 689 RSC.
  20. P. W. Kirlew, J. A. Caruso and M. T. M. Castillano, Spectrochim. Acta, Part B, in the press Search PubMed.
  21. V. Majidi, presented at the 43rd International Conference on Analytical Sciences and Spectroscopy, Montreal, Canada, August 1997, paper number 12.5.
  22. V. Majidi and N. J. Miller-Ihli, submitted for publication.
  23. L. Wang, S. W. May, R. F. Browner and S. H. Pollock, J. Anal. At. Spectrom., 1996, 11, 1137 RSC.
  24. M. Hugener, A. P. Tinke, W. M. A. Niessen, U. R. Tjaden and J. van der Greef, J. Chromatogr., 1993, 647, 375 CrossRef CAS.
  25. Handbook of Capillary Electrophoresis, ed. Landers, J. T., CRC Press, New York, 2nd edn., 1997, pp. 2–47 Search PubMed.
  26. F. Foret, S. Fanali, A. Nardi and P. Bocek, Electrophoresis, 1990, 11, 780 CAS.
  27. A. Weston, P. R. Brown, P. Jandik, W. R. Jones and A. L. Heckenberg, J. Chromatogr., 1992, 593, 289 CrossRef CAS.
  28. P. Kirlew and J. A. Caruso, Appl. Spectrosc., 1995, 52, 770 CrossRef.
Click here to see how this site uses Cookies. View our privacy policy here.