Arsenobetaine and other arsenic compounds in the National Research Council of Canada Certified Reference Materials DORM 1 and DORM 2

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Walter Goessler, Doris Kuehnelt, Claudia Schlagenhaufen, Zdenka Slejkovec and Kurt J. Irgolic


Abstract

A silica-based cation-exchange column was used to determine the arsenic compounds in the National Research Council of Canada (NRCC) CRMs DORM 1 and DORM 2 (Dogfish Muscle). With a 20 mM aqueous pyridine mobile phase at pH 3.0, the concentration of arsenobetaine was only 10.7 mg kg–1 As in the extract of DORM 1. When the same extract was chromatographed on an anion-exchange column, 15.9±0.3 mg kg–1 As (arsenobetaine) were found. The calibration for arsenobetaine was linear from 0.5 µg dm–1 As to 10 mg dm–3 As. When the extracts were diluted with water the cation-exchange results approached the anion-exchange results. The multi-element capabilities of ICP-MS allowed the simultaneous monitoring of arsenic and alkali metals. Sodium and potassium were found to co-elute with arsenobetaine. When aqueous solutions of arsenobetaine with 250 mg dm–3 Na were chromatographed, the signal obtained for arsenobetaine was only 60% of the signal without sodium in the solution. When the pH of the 20 mM aqueous pyridine mobile phase was lowered, the alkali metals were separated from arsenobetaine and the results obtained from cation-exchange chromatography were not significantly different from the anion-exchange results. Because DORM 1 is no longer available, the arsenic compounds in DORM 2 were determined. No significant difference was found for the concentration of arsenobetaine (15.6±0.7 mg kg–1 As for DORM 1; 16.0±0.7 mg kg–1 As for DORM 2). The concentrations of the minor arsenic compounds (dimethylarsinic acid, arsenocholine, the tetramethylarsonium cation and an unknown arsenic compound) in DORM 2 were only half the concentrations in DORM 1.


References

  1. T. Kaise and S. Fukui, Appl. Organomet. Chem., 1992, 6, 155 CrossRef CAS.
  2. K. A. Francesconi and J. S. Edmonds, Oceanogr. Mar. Biol. Rev., 1993, 31, 111 Search PubMed.
  3. K. J. Irgolic, in Hazardous Metals in the Environment, ed. Stoeppler, M., Elsevier, Amsterdam, 1992, pp. 287–350 Search PubMed.
  4. D. Beauchemin, M. E. Bednas, S. S. Berman, J. W. McLaren, K. W. M. Siu and R. E. Sturgeon, Anal. Chem., 1988, 60, 2209 CrossRef CAS.
  5. E. H. Larsen, G. Pritzl and S. H. Hansen, J. Anal. At. Spectrom., 1993, 8, 1075 RSC.
  6. Y. Shibata and M. Morita, Anal. Chem., 1989, 61, 2118.
  7. N. Ybanez, D. Velez, W. Tejedor and R. Montoro, J. Anal. At. Spectrom., 1995, 10, 459 RSC.
  8. G. Kölbl, K. Kalcher and K. J. Irgolic, J. Autom. Chem., 1993, 15, 37 Search PubMed.
  9. D. Kuehnelt, W. Goessler and K. J. Irgolic, Appl. Organomet. Chem., 1997, 11, 289 CrossRef CAS.
  10. D. Kuehnelt, W. Goessler and K. J. Irgolic, Appl. Organomet. Chem., 1997, 11, 459 CrossRef CAS.
  11. S. Branch, L. Ebdon and P. O'Neill, J. Anal. At. Spectrom., 1994, 9, 33 RSC.
  12. D. Velez, N. Ybanez and R. Montoro, J. Anal. At. Spectrom., 1997, 12, 91 RSC.
  13. K. J. Lamble and S. J. Hill, Anal. Chim. Acta, 1996, 334, 261 CrossRef CAS.
  14. J. J. Corr, J. Anal. At. Spectrom., 1997, 12, 537 RSC.
  15. J. A. Olivares and R. S. Houk, Anal. Chem., 1986, 58, 20 CrossRef CAS.
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