Emergence of isoperichoric focusing in multicomponent colloidal particle dispersion at sedimentation equilibrium

(Note: The full text of this document is currently only available in the PDF Version )

Josef Janča, Nathalie Caron and Natalia Gospodinova


Abstract

It has been demonstrated experimentally that the focusing effect can appear in a multicomponent (polydisperse in size) colloidal particle dispersion at sedimentation equilibrium. Consequently, an equilibrium concentration distribution of sedimenting colloidal particles of different sizes cannot be a simple convolution (or superposition) of the corresponding distributions of uniform size components of the dispersion. Although an extensive theoretical approach considering the microscopic scale interactions of the molecules or colloidal particles of the complex, gradient forming, liquid is needed to elucidate the macroscopically observed phenomena, the experimental results, reproduced several times, prove incontrovertibly the emergence of isoperichoric focusing (which is the more accurate term, compared with isopycnic focusing, describing the observed phenomena).


References

  1. A. Einstein, Ann. Phys. (Leipzig), 1906, 19, 371 Search PubMed.
  2. J. Perrin, C. R. Acad. Sci., 1908, 146, 967 Search PubMed.
  3. A. Vrij, J. Chem. Phys., 1980, 72, 3735 CrossRef CAS.
  4. M. Meselson, F. W. Stahl and J. Vinograd, Proc. Natl. Acad. Sci. USA, 1957, 43, 581 CAS.
  5. J. Montgolfier and E. Montgolfier, Description des expériences aérostatiques de MM. de Montgolfier, et de celles auxquelles cette découverte a donné lieu, Cuchet, Paris, 1784 Search PubMed.
  6. H. Svensson, Acta Chem. Scand., 1961, 15, 325 CAS.
  7. J. Janča and R. Audebert, Int. Symp. Polym. Anal. Charact., Inuyama, Japan, 1992 Search PubMed; J. Appl. Polym. Sci., Appl. Polym. Symp., 1993, 52, 63 Search PubMed.
  8. A. Kolin, in Electrofocusing and Isotachophoresis, ed. B. J. Radola and D. Graesslin, de Gruyter, Berlin, 1977 Search PubMed.
  9. J. Janča, Mikrochim. Acta, 1994, 112, 197 CAS.
  10. J. Janča and M. Špírková, Collect. Czech. Chem. Commun., 1996, 61, 819 CrossRef CAS.
  11. J. Janča, J. Colloid Interface Sci., 1997, 189, 51 CrossRef CAS.
  12. J. Janča and M. Špírková, J. Colloid Interface Sci., 1996, 184, 181 CrossRef CAS.
  13. J. Janča and M. Špírková, J. Chem. Soc., Faraday Trans., 1997, 93, 3137 RSC.
  14. D. M. E. Theis-Weesie, A. P. Philipse and H. N. W. Lekkerkerker, J. Colloid. Interface Sci., 1996, 177, 427 CrossRef.
  15. Analytical Ultracentrifugation in Biochemistry and Polymer Science, ed. S. E. Harding, J. C. Horton and A. J. Rowe, Royal Society of Chemistry, Cambridge, 1992 Search PubMed.
Click here to see how this site uses Cookies. View our privacy policy here.