Synthesis of a biotin functionalized pyrrole and its electropolymerization: toward a versatile avidin biosensor

Abstract

The synthesis of a biotinylated pyrrole and its copolymerization with pyrrole for the purpose of constructing an electronic conducting polymer (ECP) avidin sensor is reported, using fluorescence detection to determine the efficiency of the sensing process.

References

1. G. Bidan, in Polymer Films in Sensor Applications, ed. G. Harsanyi, Technomic, Pennsylvania, 1995, pp. 206–260.
2. H. K. Youssoufi, M. Hmyene, F. Garnier and D. Delabouglise, J. Chem. Soc., Chem. Commun., 1993, 1550.
3. F. Garnier, H. K. Youssoufi, P. Srivastava and A. Yassar, J. Am. Chem. Soc., 1994, 116, 8813.
4. A. Emge and P. Bäuerle, Synth. Met., 1997, 84, 213.
5. T. Livache, A. Roget, E. Dejean, C. Barthet, G. Bidan and R. Téoule, Nucleic Acids Res., 1994, 22, 2915.
6. K. Faïd and M. Leclerc, Chem. Commun., 1996, 2761.
7. T. Livache, B. Fouque, A. Roget, J. Marchand, G. Bidan, R. Téoule and G. Mathis, Anal. Biochem., 1998, 255, 188.
8. H. K. Youssoufi, F. Garnier, P. Srivastava, P. Godillot and A. Yassar, J. Am. Chem. Soc., 1997, 119, 7388.
9. D. J. Brigati, D. Myerson, J. J. Leary, B. Spalholz, S. Z. Travis, C. K. Y. Fong, G. D. Hsiung and D. C. Ward, Virology, 1983, 126, 32.
10. I. Jirkowsky and R. Baudy, Synthesis, 1981, 481.
11. H. Ge and G. G. Wallace, J. Liq. Chromatogr., 1990, 13, 3260.
12. A. Hodgson, M. Spencer and G. G. Wallace, React. Polym., 1992, 18, 77.