An efficient chemical method for removing N-terminal extra methionine from recombinant methionylated human growth hormone

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Osamu Nishimura, Masato Suenaga, Hiroaki Ohmae, Shinji Tsuji, Masato Suenaga and Masahiko Fujino


Abstract

Conversion of recombinant methionylated human growth hormone (Met-hGH) into its non-methionylated form (hGH) has been successfully achieved by a chemical process, which consists of a transamination reaction and phenylene-1,2-diamine treatment.


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